Anticancer Effects of Vitis vinifera L. Mediated Biosynthesized Silver Nanoparticles and Cotreatment with 5 Fluorouracil on HT-29 Cell Line

• Published in: Biological trace element research Vol. 200; no. 7; pp. 3159 - 3170
• Main Authors: Salman, Giray, Pehlivanoglu, Suray, Aydin Acar, Cigdem, Yesilot, Sukriye
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.07.2022; Springer Nature B.V
• Subjects: 5-Fluorouracil; Analytical methods; Anticancer properties; Antioxidants; Antiproliferatives; Apoptosis; Biochemistry; Biomedical and Life Sciences; Biotechnology; Bromodeoxyuridine; Cancer; Caspase-3; Colorectal cancer; Cytotoxicity; Dosage; Electron microscopy; Life Sciences; Nanoparticles; Nutrition; Oncology; Oxidants; Oxidative stress; Oxidizing agents; p53 Protein; Proteins; Scanning electron microscopy; Silver; Spectrophotometers; Vitis vinifera; Western blotting; X-ray spectroscopy; Green synthesis; HT-29 cell line; Colon cancer; 5-Fluorouracil; Silver nanoparticles
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-021-02923-8

The aim of this study was to evaluate the anticancer effects of biosynthesized silver nanoparticles (Vv-AgNPs) from grape ( Vitis vinifera L. ) seed aqueous extract, alone or in combination with 5-Fluorouracil (5-FU) on HT-29 cell line. Vv-AgNPs were characterized by techniques such as UV–vis spectrophotometer (surface plasmon peak 454 nm), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). HT-29 cells were treated with different concentrations (0–80 μg/mL for MTT) and (0–20 μg/mL for BrdU) of Vv-AgNPs alone and combined with (200 μg/mL) 5-FU for 72 h. The cytotoxic effects were analyzed by [3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyl tetrazolium bromide] (MTT) assay (IC 50 values 13.74 and 5.35 μg/mL, respectively). Antiproliferative effects were examined 5-bromo-2'-deoxyuridine (BrdU) assay (IC 50 values 9.65 and 5.00 μg/mL, respectively). Activation of caspase-3 and protein expression levels of p53 were determined by Western blotting analysis. It was observed that Vv-AgNPs significantly increased the cleavage of the proapoptotic proteins caspase 3 and obviously enhanced the expression of p53 in a dose-dependent manner. The increased amount of total oxidant status (TOS) in the 10 μg/mL Vv-AgNPs + 5-FU treatment group, despite the increasing amount of total antioxidant status (TAS), caused an increase in Oxidative Stress Index (OSI) compared to the control. In this study, it has been shown in vitro that the use of successfully biosynthesized Vv-AgNPs in combination with 5-FU exhibits synergistic cytotoxic, antiproliferative, apoptotic, and oxidative effects against HT-29 cell line.