LncRNA SNHG7 sponges miR-449a to promote pituitary adenomas progression

• Published in: Metabolic brain disease Vol. 36; no. 1; pp. 123 - 132
• Main Authors: Yue, Xiongfei, Dong, Ce, Ye, Zhanying, Zhu, Lin, Zhang, Xiaoyang, Wang, Xiaoyan, Mo, Feng, Li, Zheng, Pan, Baogen
• Format: Journal Article
• Language: English
• Published: New York Springer US 2021; Springer Nature B.V
• Subjects: Adenoma; Annexin V; Apoptosis; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell migration; Cell viability; Cholecystokinin; Flow cytometry; Metabolic Diseases; Neurology; Neurosciences; Oncology; Original Article; Pituitary; siRNA; Tumors; Wound healing; Xenografts; Xenotransplantation; miR-449a; Xenograft tumor; SNHG; Pituitary adenoma
• ISSN: 0885-7490; 1573-7365
• DOI: 10.1007/s11011-020-00611-5

This study aimed to characterize the expression status and potentially mechanistic involvement of SNHG7 in pituitary adenoma. Relative expression of SNHG7 and miR-449a was analyzed by real-time PCR. Cell viability was measured with Cell Counting Kit-8 (CCK-8). Cell apoptosis was determined by PI/Annexin V double staining followed by flow cytometry analysis. Cell invasion and migration were analyzed by wound healing and transwell assays, respectively. The regulatory action of miR-449a on SNHG7 was interrogated by luciferase reporter assay. We also investigated the pro-tumor activity of SNHG7 with the MMQ xenograft tumor mouse model. We identified the aberrant up-regulation of SNHG7 in pituitary adenoma both in vivo and in vitro, which associated with poor survival outcome. siRNA-mediated SNHG7-knockdown decreased cell viability, increased apoptosis and compromised migration and invasion. We further predicted and validated that SNHG7 negatively regulated miR-449a via sponging. Concurrent inhibition of miR-449a restored cell viability, apoptosis, migration and invasion influenced by SNHG7-deficiency. Most importantly, we demonstrated that SNHG7-silencing delayed xenograft tumor progression, which was accompanied with increased miR-449a and decreased Ki67 intensity. Our study highlighted the essential oncogenic properties of the SNHG7/miR-449a axis in pituitary adenoma.