Phycobiliproteins extract from Spirulina protects against single-dose cadmium-induced reproductive toxicity in male mice

• Published in: Environmental science and pollution research international Vol. 29; no. 12; pp. 17441 - 17455
• Main Authors: Montaño-González, Ricardo Iván, Gutiérrez-Salmeán, Gabriela, Mojica-Villegas, María Angélica, Cristóbal-Luna, José Melesio, Briseño-Bugarín, Jorge, Chamorro-Cevallos, Germán
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.03.2022; Springer Nature B.V
• Subjects: Animals; Antioxidants; Antioxidants - metabolism; Antioxidants - pharmacology; Apoptosis; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Cadmium; Cadmium - metabolism; Catalase; Catalase - metabolism; Damage; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Enzymatic activity; Enzyme activity; Enzyme-linked immunosorbent assay; Enzymes; Fertility; Glutathione; Glutathione peroxidase; Heavy metals; Histology; Hypogonadism; Male; Males; Malondialdehyde; Mice; Necrosis; Oxidative Stress; Peroxidase; Phycobiliproteins; Phycobiliproteins - metabolism; Phycobiliproteins - pharmacology; Plant Extracts - pharmacology; Research Article; Seminiferous tubule; Sperm; Spermatozoa; Spirulina; Spirulina - metabolism; Superoxide Dismutase - metabolism; Testes; Testis; Testosterone; Toxicity; Waste Water Technology; Water Management; Water Pollution Control; Male reproductive toxicity; Cadmium; Oxidative stress; Sperm quality; Phycobiliproteins; Mouse
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-021-16668-3

Cadmium (Cd) is known for its many toxic effects on male population such as hypogonadism and fertility difficulties, which are oftenly associated with oxidative stress. As beneficial food, Spirulina (Sp) has been proved efficient against the heavy metal toxicity. This capacity can be associated with its phycobiliproteins (PBP). In this study, the capability of PBP and Sp to treat Cd-induced oxidative damage on the testes and spermatozoa was considered. CD-1 strain mice were orally treated with either Sp or PBP for 10 days prior to single-dose Cd challenge. Sperm quality determinations and testicle histology analysis were performed. Testosterone on serum was measured using enzyme-linked immunosorbent assay (ELISA). Oxidative damage was determined. Antioxidant enzyme activity was analyzed by measuring the activity of super oxide dismutase (SOD), catalase (Cat), and glutathione peroxidase (GpX). The motility and viability of sperm decrease with Cd and improve with PBP and Sp, as the acrosomal reaction (AR) is diminished by PBPs. Testosterone levels decrease due to Cd, and only Sp maintains elevated levels. Cd increases the production of malondialdehyde in the spermatozoa, but not in testes; this production of malondialdehyde in the spermatozoa decreases in the presence of PBP. ROS only decreases with Cd, FBP, and Sp at high concentrations. Advanced oxidative protein products (AOPP) decrease with Cd and PBPs. Cat and GpX increase their activity with Cd and are altered by FBP. Cd produces vascular alterations testes. Within the seminiferous tubule, it produces areas of necrosis and apoptosis, which improve with PBPs and Sp. PBPs have a strong antioxidant activity as they show protective properties against Cd oxidative–induced toxicity on testes and sperm.