Analysis of ISG15-Modified Proteins from A549 Cells in Response to Influenza Virus Infection by Liquid Chromatography-Tandem Mass Spectrometry

• Published in: Fēnxī huàxué Vol. 44; no. 6; pp. 850 - 856
• Main Authors: PENGa, Qi-Sheng, LIb, Guang-Pu, SUNa, Wan-Chun, YANGc, Jing-Bo, QUANc, Gui-Hua, LIUc, Ning
• Format: Journal Article
• Language: English
• Published: 01.06.2016
• Subjects: Chromatography; Enzymes; Influenza; Influenza virus; Kinases; Liquids; Mass spectrometry; Proteins; Viruses
• ISSN: 0253-3820
• DOI: 10.1016/S1872-2040(16)60936-2

Interferon stimulated gene 15 kDa protein (ISG15) is the first ubiquitin-like protein identified, which plays vital roles in a variety of fields including viral infection and immunological regulation. In this study, liquid chromatography-tandem mass spectrometry was used to analyze ISG15-modified proteins in A549 cells in response to infection by influenza virus, which were enriched by immunoprecipitation. A total of 22 cellular host proteins were identified in A549 cells infected by influenza virus, including ubiquitin-like ISG15 protein, cyclin-T1, heat shock protein 71 kDa, caldesmon, eukaryotic translation initiation factor, and so on. Besides, non-structural protein (NS1) from influenza virus was also identified. Among the 22 host proteins identified, 6 were also identified in the control non-infected A549 cells, including annexin A1, fructose-bisphosphate aldolase A, ATP synthase subunit g, enolase, actin, and tubulin. Bioinformatics analysis revealed that the identified ISG15-modified host proteins induced by influenza virus infection could be classified into 9 protein classes: chaperone, oxidoreductase, enzyme modulator, transferase, nucleic acid binding, transcription factor, kinase, cytoskeletal protein, and structural protein. This study provides a specific and effective tool for analyzing ISG15-modified proteins at proteome level.