Label-free quantification of differentially expressed proteins in mouse liver cancer cells with high and low metastasis rates by a SWATH acquisition method

Label-free quantification is a valuable tool for the analysis of differentially expressed proteins identified by mass spectrometry methods. Herein, we used a new strategy： data-dependent acquisition mode identification combined with label-free quantifica- tion by SWATH acquisition mode, to study the...

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Bibliographic Details
Published inScience China. Chemistry Vol. 57; no. 5; pp. 718 - 722
Main Authors Yan, ZiQi, Zhou, Yuan, Shan, YiChu, Wu, Qi, Zhang, Shen, Liang, Zhen, Zhang, LiHua, Zhang, YuKui
Format Journal Article
LanguageEnglish
Published Heidelberg Science China Press 01.05.2014
Springer Nature B.V
Subjects
Biological properties
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Labels
Mass spectrometry
Proteins
小水线面
小鼠
差异表达
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肝癌细胞
蛋白质组
转移率
SWATH acquisition
liver cancer
differentially expressed protein
metastasis rate
label-free quantification
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Summary:Label-free quantification is a valuable tool for the analysis of differentially expressed proteins identified by mass spectrometry methods. Herein, we used a new strategy： data-dependent acquisition mode identification combined with label-free quantifica- tion by SWATH acquisition mode, to study the differentially expressed proteins in mouse liver cancer metastasis cells. A total of 1528 protein groups were identified, among which 1159 protein groups were quantified and 249 protein groups were ob- served as differentially expressed proteins （86 proteins up-regulated and 163 down-regulated）. This method provides a com- mendable solution for the identification and quantification of differentially expressed proteins in biological samples.
Bibliography:label-free quantification, SWATH acquisition, differentially expressed protein, liver cancer, metastasis rate
Label-free quantification is a valuable tool for the analysis of differentially expressed proteins identified by mass spectrometry methods. Herein, we used a new strategy： data-dependent acquisition mode identification combined with label-free quantifica- tion by SWATH acquisition mode, to study the differentially expressed proteins in mouse liver cancer metastasis cells. A total of 1528 protein groups were identified, among which 1159 protein groups were quantified and 249 protein groups were ob- served as differentially expressed proteins （86 proteins up-regulated and 163 down-regulated）. This method provides a com- mendable solution for the identification and quantification of differentially expressed proteins in biological samples.
11-5839/O6
ISSN:1674-7291
1869-1870
DOI:10.1007/s11426-014-5093-z