Cholesterol-lowering activity of adzuki bean (Vigna angularis) polyphenols

• Published in: Molecular biology reports Vol. 50; no. 7; pp. 5575 - 5584
• Main Authors: Kuriya, Kenji, Goto, Sayaka, Kobayashi, Erina, Nishio, Masahiro, Nakamura, Masahiro, Umekawa, Hayato
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.07.2023; Springer Nature B.V
• Subjects: Acids; Animal Anatomy; Animal Biochemistry; Beans; Bile acids; Biomedical and Life Sciences; Catechin; Cholesterol; Cytotoxicity; Epicatechin; Histology; Life Sciences; Morphology; mRNA; Original Article; Polyphenols; Solubility; Vigna angularis; Caco-2; -; Epicatechin 7; glucopyranoside; Cholesterol; Catechin 7; Vigna angularis; Epicatechin 7-O-β-D-glucopyranoside; Catechin 7-O-β-D-glucopyranoside
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-023-08481-7

Background Adzuki beans (ABs; Vigna angularis ) were reported to show potential for prevention of cholesterol absorption and lowering of the blood cholesterol level. However, the main active compounds and some cellular effects remain unknown. In this study, we evaluated the potential cholesterol-lowering effects of (+)-catechin 7- O - β - d -glucopyranoside (C7G) and (+)-epicatechin 7- O - β - d -glucopyranoside (E7G), identified as abundant polyphenols in ABs. Methods and results To investigate the cholesterol-lowering activity in vitro , cholesterol micelles, bile acids, and Caco-2 cells as an intestinal model were used in the study. C7G and E7G each inhibited micellar solubility in a dose-dependent manner, and their inhibitory activity was as strong as that of (+)-catechin (IC 50 values: C7G, 0.23 ± 0.03 mg/ml; E7G, 0.22 ± 0.02 mg/ml; (+)-catechin, 0.26 ± 0.11 mg/ml). The AB polyphenols showed binding activity toward bile acids and changed them into an insoluble form. When Caco-2 cells were treated with C7G or E7G, the amount of incorporated cholesterol was significantly decreased compared with vehicle-treated control cells, and no cytotoxicity was observed under the experimental conditions used. Meanwhile, quantitative real-time PCR revealed that the mRNA level of the cholesterol transporter NPC1L1 remained unchanged in the treated cells. Conclusions Taken together, the present findings suggest that C7G and E7G are the main active compounds in ABs, and have the ability to inhibit micellar solubility, bind to bile acids, and suppress cholesterol absorption. The present study supports the health benefits of ABs as a medicinal food and the application of AB polyphenols as medicinal supplements to suppress cholesterol elevation. Graphical abstract