Bactericidal Action of Green Tea Extract and Damage to the Membrane of Escherichia coli O157: H7

The antibacterial activity of green tea extract was investigated on 36 isolates of pathogens. The minimum inhibitory concentration of the extract against some isolates such as Escherichia coli O157: H7 was less than 250 μg/ml. The growth of the organism in culture broth was completely inhibited with...

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Bibliographic Details
Published inBiocontrol Science Vol. 6; no. 1; pp. 57 - 61
Main Authors HARA-KUDO, YUKIKO, OKUBO, TSUTOMU, TANAKA, SHIN, CHU, DJONG-CHI, JUNEJA, LEKH RAJ, SAITO, NORIKO, SUGITA-KONISHI, YOSHIKO
Format Journal Article
LanguageEnglish
Published The Society for Antibacterial and Antifungal Agents, Japan 2001
Subjects
Bactericidal action
chatechin
E. coli O157: H7
Escherichia coli
Green tea
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Summary:The antibacterial activity of green tea extract was investigated on 36 isolates of pathogens. The minimum inhibitory concentration of the extract against some isolates such as Escherichia coli O157: H7 was less than 250 μg/ml. The growth of the organism in culture broth was completely inhibited with 500 μg/ml of the extract. The organism was not detected by culturing on agar plates after 6 h incubation in phosphate buffered saline with 1, 000 μg/ml of the extract. It was observed by fluorescence microscopy and scanning electric microscopy that the cell membranes were damaged with 1, 000 μg/ml of the extract.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1342-4815
1884-0205
DOI:10.4265/bio.6.57