Overexpression of PsoRPM3, an NBS-LRR gene isolated from myrobalan plum, confers resistance to Meloidogyne incognita in tobacco

• Published in: Plant molecular biology Vol. 107; no. 3; pp. 129 - 146
• Main Authors: Xiao, Kun, Zhu, Haifeng, Zhu, Xiang, Liu, Zhenhua, Wang, Yan, Pu, Wenjiang, Guan, Pingyin, Hu, Jianfang
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.10.2021; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Cloning, Molecular; Disease Resistance - genetics; DNA Methylation; Gene expression; Gene Expression Regulation, Plant; Hypersensitive response; Infections; Jasmonic acid; Life Sciences; Meloidogyne incognita; NBS-LRR gene; Nicotiana - genetics; Nicotiana - immunology; Nicotiana - parasitology; Pest resistance; Phylogeny; Plant Diseases - genetics; Plant Diseases - parasitology; Plant Immunity - genetics; Plant Pathology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant resistance; Plant Sciences; Plants, Genetically Modified; Promoter Regions, Genetic; Prunus - genetics; Prunus sogdiana; RNA-mediated interference; Salicylic acid; Tobacco; Transgenic plants; Tylenchoidea - pathogenicity; Hypersensitive response; Plant defence; Methylation; gene; Myrobalan plum; Meloidogyne incognita; PsoRPM3 gene
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/s11103-021-01185-1

Key messages We reported an NBS-LRR gene, PsoRPM3 , is highly expressed following RKN infection, initiating an HR response that promotes plant resistance. Meloidogyne spp. are root-knot nematodes (RKNs) that cause substantial economic losses worldwide. Screening for resistant tree resources and identifying plant resistance genes is currently the most effective way to prevent RKN infestations. Here, we cloned a novel TIR-NB-LRR-type resistance gene, PsoRPM3 , from Xinjiang wild myrobalan plum ( Prunus sogdiana Vassilcz.) and demonstrated that its protein product localized to the nucleus. In response to Meloidogyne incognita infection, PsoRPM3 gene expression levels were significantly higher in resistant myrobalan plum plants compared to susceptible plants. We investigated this difference, discovering that the − 309 to − 19 bp region of the susceptible PsoRPM3 promoter was highly methylated. Indeed, heterologous expression of PsoRPM3 significantly enhanced the resistance of susceptible tobacco plants to M. incognita . Moreover, transient expression of PsoRPM3 induced a hypersensitive response in tobacco, whereas RNAi-mediated silencing of PsoRPM3 in transgenic tobacco reduced this hypersensitive response. Several hypersensitive response marker genes were considerably up-regulated in resistant myrobalan plum plants when compared with susceptible counterparts inoculated with M. incognita . PsoPR1a (a SA marker gene), PsoPR2 (a JA marker gene), and PsoACS6 (an ET signaling marker gene) were all more highly expressed in resistant than in susceptible plants. Together, these results support a model in which PsoRPM3 is highly expressed following RKN infection, initiating an HR response that promotes plant resistance through activated salicylic acid, jasmonic acid, and ethylene signaling pathways.