Autotransplantation of the Adipose Tissue-Derived Mesenchymal Stromal Cells in Therapy of Venous Stasis Ulcers

• Published in: Archivum Immunologiae et Therapiae Experimentalis Vol. 68; no. 1; p. 5
• Main Authors: Masłowski, Leszek, Paprocka, Maria, Czyżewska-Buczyńska, Agnieszka, Bielawska-Pohl, Aleksandra, Duś, Danuta, Grendziak, Ryszard, Witkiewicz, Wojciech, Czarnecka, Anna
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 14.02.2020; Springer Nature B.V
• Subjects: Adipose tissue; Autografts; Biomedical and Life Sciences; Biomedicine; CD34 antigen; CD73 antigen; CD90 antigen; Immunology; Mesenchymal stem cells; Mesenchyme; Original Article; Pharmacology/Toxicology; Phenotypes; Regenerative medicine; Stromal cells; Transplantation; Ulcers; Wounds; Venous stasis ulcers; Wound healing; Adipose tissue-derived mesenchymal stromal cells; CELLUTION 800; Regenerative medicine
• ISSN: 0004-069X; 1661-4917
• DOI: 10.1007/s00005-020-00571-9

Adipose tissue is a reliable source of mesenchymal stromal cells (MSC) for use in regenerative medicine. The aim of this pilot study was to describe the method, and assess the safety and the potential efficacy of transplantation of autologous adipose tissue-derived MSC for the treatment of chronic venous stasis ulcers. Study group consisted of 11 patients (mean age: 66.6 ± 9.5 years) with chronic venous stasis ulcers. Adipose tissue was harvested by tumescent-aspiration method. Stromal cells were separated using a dedicated closed system in a real-time bedside manner. The phenotype of cells was determined immediately after separation. Cell concentrate was implanted subcutaneously around the wound and the wound bed. All ulcers were assessed planimetrically before autotransplantation and every two weeks during the six-month follow-up. During the study all patients received standard local and general treatment. The preparation contained an average of 5.6 × 10 6  ± 4 × 10 6 cells per milliliter. The phenotype of 65–82% of transplanted cells expressed MSC markers: CD73 + CD90 + and CD34 + . An improvement was observed in 75% of ulcers. The data showed highly significant negative correlation ( p  < 0.0001) between wound size and wound closure degree. There was no correlation of ulcer healing with other parameters evaluated, including age of the patients. No serious side effects were observed. Autotransplantation of adipose tissue stromal cells may be a safe and promising treatment method for chronic venous ulcers.