Moraxella haemolytica sp. nov., isolated from a goat with respiratory disease

• Published in: Archives of microbiology Vol. 206; no. 1; p. 45
• Main Authors: Li, Fu-Xiang, Zhao, Wen-Hua, Li, Zhan-Hong, Song, Jian-Ling, Gao, Hua-Feng
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2024; Springer Nature B.V
• Subjects: Amino acids; Animals; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cardiolipin; Cell Biology; Deoxyribonucleic acid; DNA; Ecology; Gene sequencing; Genomes; Goats; Hybridization; Life Sciences; Lipids; Microbial Ecology; Microbiology; Moraxella; Moraxella - genetics; New species; Nucleotide sequence; Nucleotides; Original Paper; Phosphatidic acid; Phosphatidylethanolamine; Phosphatidylglycerol; Phylogenetics; Phylogeny; Quinones; Respiratory diseases; Respiratory Tract Diseases; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sheep; Similarity; Sodium chloride; Haemolysis; Goat; Novel species; Respiratory disease; Moraxella haemolytica
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-023-03782-8

An aerobic, haemolytic, Gram-negative and rod-shaped bacterial strain ZY171148 T was isolated from the lung of a dead goat with respiratory disease in Southwest China. The strain grew at 24–39 °C, at pH 6.0–9.0 and in the presence of 0.5–2.0% (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain belongs to the genus Moraxella . The nucleotide sequence similarity analysis of the 16S rRNA gene showed that the strain has the highest similarity of 98.1% to Moraxella ( M .) caprae ATCC 700019  T . Phylogenomic analysis of 800 single-copy protein sequences indicated that the strain is a member of the genus Moraxella and forms a separated branch on the Moraxella phylogenetic tree. The strain exhibited the highest orthologous average nucleotide identity (OrthoANI) and average amino acid identity (AAI) values of 77.0 and 77.9% to M . nasibovis CCUG 75921 T and M . ovis CCUG 354 T , respectively. The strain shared the highest digital DNA-DNA hybridization (dDDH) value of 26.2% to M . osloensis CCUG 350 T . The genome G + C content of strain ZY171148 T was 42.6 mol%. The strain had C 18:1 ω9c (41.7%), C 18:0 (11.2%), C 16:0 (14.1%) and C 12:0 3OH (9.7%) as the predominant fatty acids and CoQ-8 as the major respiratory quinone. The strain contained phosphatidylglycerol, phosphatidylethanolamine, cardiolipin, dilysocardiolipin, monolysocardiolipin and phosphatidic acid as the major polar lipids. β-haemolysis was observed on Columbia blood agar. All results confirmed that strain ZY171148 T represents a novel species of the genus Moraxella , for which the name Moraxella haemolytica sp. nov. is proposed, with strain ZY171148 T  = CCTCC AB 2021471 T  = CCUG 75920 T as the type strain.