Antibacterial evaluation and molecular docking studies of pyrazole–thiosemicarbazones and their pyrazole–thiazolidinone conjugates

• Published in: Molecular diversity Vol. 25; no. 1; pp. 191 - 204
• Main Authors: Ebenezer, Oluwakemi, Singh-Pillay, Ashona, Koorbanally, Neil A., Singh, Parvesh
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.02.2021; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Gram-positive bacteria; Life Sciences; Organic Chemistry; Original Article; Pharmacy; Polymer Sciences; Staphylococcus infections; Molecular hybridization; Thiazolidinones; Antibacterial; Pyrazoles; Molecular docking
• ISSN: 1381-1991; 1573-501X
• DOI: 10.1007/s11030-020-10046-w

A library of pyrazole–thiazolidinone conjugates was synthesized using a molecular hybridization approach through a Vilsmeier–Haack reaction. The compounds were tested for anti-microbial activity against two Gram-positive bacteria ( Staphylococcus aureus and methicillin-resistant Staphylococcus aureus ) and four Gram-negative bacteria ( Escherichia coli , Salmonella typhimurium , Klebsiella pneumonia and Pseudomonas aeruginosa ). Among the compounds tested , 3 - ((2,4 - dichlorophenyl) - 1 - (2,4 - dinitrophenyl) - 1H - pyrazol-yl)methylene)hydrazinecarbothioamide ( 3a ) and 2 - ((3 - (2 - chlorophenyl) - 1 - (2,4 dinitrophenyl) - 1H - pyrazol - 4 - yl)methyleneamino)thiazolidin - 4 - on e ( 4b ) emerged as the most potent anti-microbial compounds with minimum bactericidal concentrations of < 0.2 µM against MRSA and S. aureus . Structure–activity relationship analysis further revealed that the presence of 2,4-dichloro moiety surprisingly influenced the activity of the compounds. Molecular docking studies of the compounds into the crystal structure of topoisomerase II and topoisomerase IV suggest that compounds 3a and 4b preferably interact with the targets through hydrogen bonding.