Isolation, Characterization, Antioxidant, and Wound Healing Activities of Extracellular Polysaccharide from Endophytic Fungus Talaromyces purpureogenus

• Published in: Applied biochemistry and biotechnology Vol. 195; no. 6; pp. 3822 - 3839
• Main Authors: Hu, Xiaowen, Saravanakumar, Kandasamy, Park, SeonJu, Han, Ki-seok, Wang, Myeong-Hyeon
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.06.2023; Springer Nature B.V
• Subjects: Antioxidants; Antioxidants - chemistry; Biochemistry; Biotechnology; Chemistry; Chemistry and Materials Science; Chromatography; Endophytes; Fungi; Galactose; HEK293 Cells; Humans; Liquid chromatography; Mannose; NMR; Nuclear magnetic resonance; Original Article; Phenols; Polysaccharides; Polysaccharides - chemistry; Ribose; Saccharides; Scavenging; Sodium chloride; Talaromyces; Talaromyces - metabolism; Wound Healing; Structural characteristics; Antioxidant activity; Extracellular polysaccharides; Talaromyces purpureogenus
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-022-04187-x

In this study, two extracellular polysaccharides (TEPS1 and TEPS2) were isolated from the endophytic fungus ( Talaromyces purpureogenus ) and purified by DEAE-Sepharose Fast Flow column using NaCl as gradient eluent. The HPLC analysis displayed that TEPS1 was composed of mannose (38.70%), ribose (25.02%), glucose (19.34%), and galactose (16.94%) while the TEPS2 composed by mannose (100%). The NMR results indicated that TEPS1 exhibited α-glycosidic configurations. The both polysaccharides, TEPS1 and TEPS2 were exhibited a good antioxidant activity in terms of DPPH, ABTS, and •OH scavenging. However, TEPS1 showed a higher antioxidant activity than TEPS2. The IC 50 of TEPS1 were 32.16, 192.57, and 54.67 μg·mL −1 , for DPPH, ABTS, and •OH radical scavenging, respectively. Furthermore, TEPS1 showed the high cellular antioxidant and wound healing activity in the human embryonic kidney (HEK293) cell line. Overall, these two polysaccharides were promising in antioxidant activity.