Comparison of six methods of DNA extraction for the diagnosis of bovine brucellosis by real-time PCR

Brucellosis is an infectious disease caused by bacteria of the genus Brucella , which affects domestic animals and is transmissible to humans. The objective of this study was to evaluate six methods of DNA extraction directly from bovine tissue to detect Brucella spp. The Cq values for all samples w...

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Bibliographic Details
Published inArchives of microbiology Vol. 201; no. 8; pp. 1025 - 1028
Main Authors Preis, Ingred Sales, Moura, André, de Souza, Patrícia Gomes, Filho, Paulo Martins Soares, Fonseca Júnior, Antônio Augusto
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2019
Springer Nature B.V
Subjects
Biochemistry
Biomedical and Life Sciences
Biotechnology
Brucella
Brucellosis
Cell Biology
Deoxyribonucleic acid
Diagnostic systems
DNA
Domestic animals
Ecology
Infectious diseases
Life Sciences
Microbial Ecology
Microbiology
Original Paper
Statistical methods
Brucellosis
DNA extraction
qPCR
Diagnosis
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Summary:Brucellosis is an infectious disease caused by bacteria of the genus Brucella , which affects domestic animals and is transmissible to humans. The objective of this study was to evaluate six methods of DNA extraction directly from bovine tissue to detect Brucella spp. The Cq values for all samples were above 30 and varied according to the extraction kit used, but four kits showed no statistical difference in sensitivity. This work demonstrates the importance of choosing the best extraction kit before validation of a molecular diagnostic technique.
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ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-019-01675-3