Assessment of the photoprotective potential and structural characterization of secondary metabolites of Antarctic fungus Arthrinium sp

• Published in: Archives of microbiology Vol. 206; no. 1; p. 35
• Main Authors: Jordão, Ana Carolina, dos Santos, Gustavo Souza, Teixeira, Thaiz Rodrigues, Gluzezak, Ana Júlia Pasuch, de Souza Azevedo, Clarissa Bechuate, de Castro Pereira, Karina, Tonani, Ludmilla, Gaspar, Lorena Rigo, von Zeska Kress, Márcia Regina, Colepicolo, Pio, Debonsi, Hosana Maria
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2024; Springer Nature B.V
• Subjects: Absorbance; Algae; Antioxidants; Antioxidants - metabolism; Antioxidants - pharmacology; Arthrinium; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Ecology; Endophytes; Formulations; Fungi; Harsh environments; Humans; Irritation; Life Sciences; Metabolites; Microbial Ecology; Microbiology; Original Paper; Orthosporin; Seaweeds; Secondary metabolites; Skin; Structural analysis; Sun screens; Sunscreening Agents - chemistry; Sunscreening Agents - pharmacology; Sunscreens; Synergistic effect; Ultraviolet Rays; Xylariales; Reconstructed human skin; Antioxidant; Photoprotection; Marine Antarctic natural products; Arthrinium
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-023-03756-w

Interest in Antarctic fungi has grown due to their resilience in harsh environments, suggesting the presence of valuable compounds from its organisms, such as those presenting photoprotective potential, since this environment suffers the most dangerous UV exposure in the world. Therefore, this research aimed to assess the photoprotective potential of compounds from sustainable marine sources, specifically seaweed-derived fungi from Antarctic continent. These studies led to discovery of photoprotective and antioxidant properties of metabolites from Arthrinium sp., an endophytic fungus from Antarctic brown algae Phaeurus antarcticus . From crude extract, fractions A-I were obtained and compounds 1–6 isolated from E and F fractions, namely 3-Hydroxybenzyl alcohol ( 1 ), (-)-orthosporin ( 2 ), norlichexanthone ( 3 ), anomalin B ( 4 ), anomalin A ( 5 ), and agonodepside B ( 6 ). Compounds 1 , 2 , and 6 were not previously reported in Arthrinium . Fraction F demonstrated excellent absorbance in both UVA and UVB regions, while compound 6 exhibited lower UVB absorbance, possibly due to synergistic effects. Fraction F and compound 6 displayed photostability and were non-phototoxic to HaCaT cells. They also exhibited antioxidant activity by reducing intracellular ROS production induced by UVA in keratinocyte monolayers and reconstructed human skin models (resulting in 34.6% and 30.2% fluorescence reduction) and did not show irritation potential in HET-CAM assay. Thus, both are promising candidates for use in sunscreens. It is noted that Fraction F does not require further purification, making it advantageous, although clinical studies are necessary to confirm its potential applicability for sunscreen formulations.