CKIP-1 Promotes P. gingivalis-Induced Inflammation of Periodontal Soft Tissues by Inhibiting Autophagy

• Published in: Inflammation Vol. 46; no. 5; pp. 1997 - 2010
• Main Authors: Xiao, Junhong, Huang, Xin, Wang, Huiyi, Peng, Yan, Liu, Heyu, Huang, Hantao, Ma, Li, Wang, Chuan, Wang, Xiaoxuan, Cao, Zhengguo
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2023; Springer Nature B.V
• Subjects: Animals; Autophagy; Biomedical and Life Sciences; Biomedicine; Carrier Proteins - metabolism; Casein; Casein kinase II; Cytokines - metabolism; Fibroblasts; Gingiva - metabolism; Gum disease; Humans; Immunology; Inflammation; Inflammation - metabolism; Inflammatory diseases; Internal Medicine; Kinases; Mice; Pathology; Periodontal ligament; Periodontitis; Periodontitis - metabolism; Pharmacology/Toxicology; Porphyromonas gingivalis - metabolism; Rapamycin; Rheumatology; Soft tissues; autophagy; CKIP-1; inflammation; periodontitis; Porphyromonas gingivalis
• ISSN: 0360-3997; 1573-2576
• DOI: 10.1007/s10753-023-01856-9

— As a chronic inflammatory disease, periodontitis involves many biological processes including autophagy. At the same time, casein kinase 2 interacting protein-1 (CKIP-1) was reported to play a role in regulation of inflammation. But whether CKIP-1 and autophagy interact in periodontitis remains unclear. In this paper, our research team verified the levels of CKIP-1 expression and autophagy increase in the periodontal tissues of a ligature-induced periodontitis mouse model. And this result was also confirmed in Porphyromonas gingivalis (Pg)-induced human gingival fibroblasts (HGF) and human periodontal ligament cells (PDLC). We also showed the autophagy level in periodontal tissues is higher in Ckip-1 knockout (KO) mice than wild type (WT). At the same time, CKIP-1 knockdown lentivirus was used in PDLC and HGF, and it was found that silencing CKIP-1 significantly activated autophagy. Unfortunately, the regulatory role of autophagy in periodontitis is still unclear. Then, the autophagy agonist Rapamycin and inhibitor 3-MA were used in a periodontitis mouse model to investigate periodontal tissue destruction. We found the inflammation in periodontal tissue was reduced when autophagy activated. All these conclusions have been verified both in vivo and in vitro experiments. Finally, our research proved that silencing CKIP-1 reduces the expression of inflammatory cytokines in Pg-induced PDLC and HGF by regulating autophagy. Overall, a new role for CKIP-1 in regulating periodontal tissue inflammation was demonstrated in our study, and it is possible to treat periodontitis by targeting the CKIP-1 gene.