LncRNA NEAT1 ameliorate ischemic stroke via promoting Mfn2 expression through binding to Nova and activates Sirt3

• Published in: Metabolic brain disease Vol. 37; no. 3; pp. 653 - 664
• Main Authors: Zhou, Zhi-Wen, Ren, Xiang, Zheng, Li-Jun, Li, Ai-Ping, Zhou, Wen-Sheng
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2022; Springer Nature B.V
• Subjects: Apoptosis; Apoptosis - genetics; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cytokines; Deprivation; Enzyme-linked immunosorbent assay; Flow cytometry; Glucose; Glucose - metabolism; GTP Phosphohydrolases - genetics; Humans; Inflammation; Ischemia; Ischemic Stroke; Metabolic Diseases; MicroRNAs - genetics; Mitochondrial Proteins - genetics; mRNA; Neurology; Neurosciences; Non-coding RNA; Oncology; Original Article; Oxidative stress; Oxidative Stress - genetics; Oxygen; Pathogenesis; Reperfusion; Reperfusion Injury; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Sirtuin 3 - genetics; Sirtuin 3 - metabolism; Stroke; Up-Regulation; LncRNA NEAT1; Sirt3; Mfn2; Nova; ischemia stroke
• ISSN: 0885-7490; 1573-7365
• DOI: 10.1007/s11011-021-00895-1

Background Recent studies revealed that long non-coding RNAs (lncRNAs) have significant roles in regulating the pathogenesis of ischemia stroke, and oxygen-glucose deprivation/reoxygenation (OGD/R)-induced cell apoptosis. Aberrant expression of NEAT1 was found after the injury of ischemia-reperfusion, but the mechanism was not fully understood. Methods The expression of NEAT1 and Mfn2 were detected in BV-2 and N2a cell with or without OGD/R-induced by qRT-PCR. Inflammatory cytokines secretion was detected by enzyme-linked immunosorbent assay (ELISA). The oxidative stress was evaluated by the examination of ROS, MDA and SOD levels. Flow cytometry and apoptosis marker detection by western blot were performed to examined apoptosis. Results The expression of NEAT1 and Mfn2 were decreased in OGD/R-induced cell model. Overexpression of NEAT1 or Mfn2 reduced oxidative stress and apoptosis by OGD/R-induced in neuronal cells, while knockdown of Sirt3 reversed the protective effect of NEAT1 and Mfn2. NEAT1 stabilized Mfn2 mRNA via recruiting Nova. NEAT1 alleviates the oxidative stress and apoptosis by OGD/R-induced via activating Sirt3. Conclusion LncRNA NEAT1 stabilizes Mfn2 mRNA via recruiting Nova, therefore increase the expression of Mfn2 and alleviates ischemia-reperfusion induced oxidative stress and apoptosis via Mfn2/Sirt3 pathway.