Enzymatic, Non-enzymatatic Antioxidant Levels and Heat Shock Protein Expression as Indicators of Metal Induced Toxicity and Reproductive Modulation in Female Indian Major Carp Cirrhinus cirrhosus

• Published in: Bulletin of environmental contamination and toxicology Vol. 104; no. 2; pp. 235 - 244
• Main Authors: Moniruzzaman, Mahammed, Das, Debjit, Dhara, Anindita, Chakraborty, Suman Bhusan
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.02.2020; Springer Nature B.V
• Subjects: 17β-Estradiol; Animals; Antioxidants; Antioxidants - metabolism; Aquatic Pollution; Biomarkers; Brain; Carp; Cirrhinus cirrhosus; Cyprinidae - metabolism; Cyprinidae - physiology; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Biomarkers - drug effects; Environmental Chemistry; Environmental Health; Enzymes; Exposure; Female; Females; Heat shock proteins; Heat-Shock Proteins - genetics; Heavy metals; Hsp70 protein; Hsp90 protein; Lead; Metal concentrations; Metals - metabolism; Metals - toxicity; Modulation; Oxidative Stress - drug effects; Pollution; Recovery; Reproduction - drug effects; Sex hormones; Soil Science & Conservation; Steroidogenesis; Toxicity; Waste Water Technology; Water Management; Water Pollutants, Chemical - metabolism; Water Pollutants, Chemical - toxicity; Water Pollution Control; Zinc; Lead; Steroidogenesis; Antioxidant; Zinc; Heat shock protein
• ISSN: 0007-4861; 1432-0800
• DOI: 10.1007/s00128-019-02766-z

Effects of zinc (Zn) and lead (Pb) exposure on oxidative biomarkers and heat shock protein (HSP) expression, and their possible relation to ovarian steroidogenesis in fish were investigated. Female Cirrhinus cirrhosus were exposed to 1/10th of LC 50 of metals for 30 days, and kept for another 30 days without metal exposure. Metal concentrations, antioxidants, HSPs, 17β-estradiol and steroidogenic enzymes were analysed in brain and ovary after 15 and 30 days of exposure and a 15 and 30 day recovery period. Activities of enzymatic and non-enzymatic antioxidants showed duration dependent variation in both exposure and recovery period. HSP70 and HSP90 expressions increased following metal exposure, with the expression being higher in brain than ovary. 17β-Estradiol, steroidogenic enzymes decreased significantly ( p  < 0.05) after metal exposure. The present study suggests that metals have differential and tissue specific influence on oxidative status and manipulate ovarian steroidogenesis probably through the modulation of HSPs.