The SMC5/6 complex subunit MMS21 regulates stem cell proliferation in rice
Key message SMC5/6 complex subunit OsMMS21 is involved in cell cycle and hormone signaling and required for stem cell proliferation during shoot and root development in rice . The structural maintenance of chromosome (SMC)5/6 complex is required for nucleolar integrity and DNA metabolism. Moreover,...
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Published in | Plant cell reports Vol. 42; no. 8; pp. 1279 - 1290 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.08.2023
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Key message
SMC5/6 complex subunit OsMMS21 is involved in cell cycle and hormone signaling and required for stem cell proliferation during shoot and root development in rice
.
The structural maintenance of chromosome (SMC)5/6 complex is required for nucleolar integrity and DNA metabolism. Moreover,
METHYL METHANESULFONATE SENSITIVITY GENE 21
(
MMS21
), a SUMO E3 ligase that is part of the SMC5/6 complex, is essential for the root stem cell niche and cell cycle transition in Arabidopsis. However, its specific role in rice remains unclear. Here,
OsSMC5
and
OsSMC6
single heterozygous mutants were generated using CRISPR/Cas9 technology to elucidate the function of SMC5/6 subunits, including
OsSMC5
,
OsSMC6
, and
OsMMS21
, in cell proliferation in rice.
ossmc5
/ + and
ossmc6
/ + heterozygous single mutants did not yield homozygous mutants in their progeny, indicating that
OsSMC5
and
OsSMC6
both play necessary roles during embryo formation. Loss of
OsMMS21
caused severe defects in both the shoot and roots in rice. Transcriptome analysis showed a significant decrease in the expression of genes involved in auxin signaling in the roots of
osmms21
mutants. Moreover, the expression levels of the
cycB2-1
and
MCM
genes, which are involved the cell cycle, were significantly lower in the shoots of the mutants, indicating that
OsMMS21
was involved in both hormone signaling pathways and the cell cycle. Overall, these findings indicate that the SUMO E3 ligase
OsMMS21
is required for both shoot and root stem cell niches, improving the understanding of the function of the SMC5/6 complex in rice. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0721-7714 1432-203X |
DOI: | 10.1007/s00299-023-03030-9 |