High internal phase Pickering emulsions stabilized by co-assembled rice proteins and carboxymethyl cellulose for food-grade 3D printing

• Published in: Carbohydrate polymers Vol. 273; p. 118586
• Main Authors: Wan, Ying, Wang, Ren, Feng, Wei, Chen, Zhengxing, Wang, Tao
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.12.2021
• Subjects: 3D printing; adhesion; Carboxymethyl cellulose; carboxymethylcellulose; chewiness; droplets; electrostatic interactions; hardness; High internal phase Pickering emulsions; hydrophilicity; hydrophobicity; oils; rice; Rice proteins; storage time; Substitution degree; surface tension; wettability; RC 0.9; Carboxymethyl cellulose; RCs; HIPEs; Rice proteins; RC 0.7; HIPPEs; RC 1.2; Substitution degree; High internal phase Pickering emulsions; DS; RPs; CMC; 3D printing; 3DP
• ISSN: 0144-8617; 1879-1344; 1879-1344
• DOI: 10.1016/j.carbpol.2021.118586

In this study, high internal phase Pickering emulsions (HIPPEs) stabilized by protein-polysaccharide complexes were used as inks for food-grade three-dimensional printing (3DP). The complexes (RCs) structured by synergistic interactions between rice proteins (RPs) and carboxymethyl cellulose (CMC) displayed outstanding biphasic wettability with excellent ability to reduce the oil/water interfacial tension. The interfacial structures formed by RCs provided a steric barrier and sufficient electrostatic repulsion, preventing droplet coalescence against heating treatment as well as long-term storage. Moreover, the rheological behaviors of the HIPPEs can be tuned by the substitution degree (DS) of CMC for tailorable hydrophobic/hydrophilic properties of RCs, allowing their controllable injectability and printability during 3DP. The HIPPEs stabilized by RCs with a DS 1.2 showed the most favorable printing resolution, hardness, adhesiveness, and chewiness. Associating the hydrophobic RPs with hydrophilic CMC, our study enabled on-demand amphiphilicity of RCs for effective stabilization of HIPPEs that can be manipulated for 3DP. •Amphiphilic protein-polysaccharide complexes (RCs) were prepared by pH-cycle.•The interfacial properties of RCs could be controlled by the DS of CMC.•High internal phase emulsions (HIPEs) were prepared by RCs with good stability.•The HIPPEs were injectable and were amenable for there-dimensional printing.