Mannitol-1-phosphate dehydrogenase, MpdA, is required for mannitol production in vegetative cells and involved in hyphal branching, heat resistance of conidia and sexual development in Aspergillus nidulans

• Published in: Current genetics Vol. 67; no. 4; pp. 613 - 630
• Main Authors: Lim, Joo-Yeon, Jang, Seung-Hyun, Park, Hee-Moon
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2021; Springer Nature B.V
• Subjects: Ascospores; Aspergillus nidulans; Biochemistry; Biomedical and Life Sciences; Cell Biology; Cleistothecia; Conidia; Dehydrogenase; Dehydrogenases; Gene deletion; Heat resistance; Hypoxia; Life Sciences; Mannitol; Mannitol-1-phosphate 5-dehydrogenase; Meiosis; Microbial Genetics and Genomics; Microbiology; Mycelia; Organs; Original Article; Phenotypes; Plant Sciences; Proteins; Proteomes; Proteomics; Reproductive organs; Thermal resistance; Transcription; Vegetative cells; Hyphal branching; Heat resistance; Sexual development; Mannitol phosphate dehydrogenase; Mannitol; Aspergillus nidulans
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/s00294-021-01163-6

Aspergillus nidulans produces cleistothecia as sexual reproductive organs in a process affected by genetic and external factors. To gain a deeper insight into A. nidulans sexual development, we performed comparative proteome analyses based on the wild type developmental periods. We identified sexual development-specific proteins with a more than twofold increase in production during hypoxia or the sexual period compared to the asexual period. Among the sexual development-specific proteins analyzed by gene-deletion experiments and functional assays, MpdA, a putative mannitol-1-phosphate 5-dehydrogenase, plays multiple roles in growth and differentiation of A. nidulans . The most distinct mpdA -deletion phenotype was ascosporogenesis failure. Genetic mpdA deletion resulted in small cleistothecia with no functional ascospores. Transcriptional analyses indicated that MpdA modulates the expression of key development- and meiosis-regulatory genes during sexual development. The mpdA deletion increased hyphal branching and decreased conidial heat resistance. Mannitol production in conidia showed no difference, whereas it was decreased in mycelia and sexual cultures. Addition of mannitol during vegetative growth recovered the defects in conidial heat resistance and ascospore genesis. Taken together, these results indicate that MpdA plays an important role in sexual development, hyphal branching, and conidial heat resistance in Aspergillus nidulans .