Fifteen days of moderate normobaric hypoxia does not affect mitochondrial function, and related genes and proteins, in healthy men

• Published in: European journal of applied physiology Vol. 121; no. 8; pp. 2323 - 2336
• Main Authors: Ferri, Alessandra, Yan, Xu, Kuang, Jujiao, Granata, Cesare, Oliveira, Rodrigo S. F., Hedges, Christopher P., Lima-Silva, Adriano E., Billaut, Francois, Bishop, David J.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2021; Springer Nature B.V
• Subjects: Adaptation; Biomedical and Life Sciences; Biomedicine; Biopsy; Biosynthesis; Citrate synthase; Electron transport; Human Physiology; Hypoxia; Mitochondria; Occupational Medicine/Industrial Medicine; Original Article; Proteins; Respiration; Skeletal muscle; Sports Medicine; Mitochondrial respiration; PGC-1α; LONP; P53
• ISSN: 1439-6319; 1439-6327
• DOI: 10.1007/s00421-021-04706-4

Purpose To investigate within the one study potential molecular and cellular changes associated with mitochondrial biogenesis following 15 days of exposure to moderate hypoxia. Methods Eight males underwent a muscle biopsy before and after 15 days of hypoxia exposure (FiO 2  = 0.140–0.154; ~ 2500–3200 m) in a hypoxic hotel. Mitochondrial respiration, citrate synthase (CS) activity, and the content of genes and proteins associated with mitochondrial biogenesis were investigated. Results Our main findings were the absence of significant changes in the mean values of CS activity, mitochondrial respiration in permeabilised fibers, or the content of genes and proteins associated with mitochondrial biogenesis, after 15 days of moderate normobaric hypoxia. Conclusion Our data provide evidence that 15 days of moderate normobaric hypoxia have negligible influence on skeletal muscle mitochondrial content and function, or genes and proteins content associated with mitochondrial biogenesis, in young recreationally active males. However, the increase in mitochondrial protease LON content after hypoxia exposure suggests the possibility of adaptations to optimise respiratory chain function under conditions of reduced O 2 availability.