Can Echinococcus granulosus-Derived MicroRNAs be Biomarkers for Diagnosis and Follow-up of Cystic Echinococcosis Patients?

Introduction Cystic echinococcosis (CE) is a neglected tropical disease caused by the larval stages of Echinococcus granulosus ( E. granulosus ). MicroRNAs (miRNAs) are small noncoding RNAs acting as mediators in host-parasite interaction. Recently, numerous studies have been conducted on miRNAs in...

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Published inActa parasitologica Vol. 68; no. 1; pp. 231 - 239
Main Authors Fasihi Karami, Maryam, Beiromvand, Molouk, Rafiei, Abdollah, Dayer, Dian, Rahdar, Mahmoud, Bahreini, Amin, Dastyar, Ali Asghar
Format Journal Article
LanguageEnglish
Published Cham Springer International Publishing 01.03.2023
Springer Nature B.V
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Summary:Introduction Cystic echinococcosis (CE) is a neglected tropical disease caused by the larval stages of Echinococcus granulosus ( E. granulosus ). MicroRNAs (miRNAs) are small noncoding RNAs acting as mediators in host-parasite interaction. Recently, numerous studies have been conducted on miRNAs in infectious diseases; however, little data are available about the role of miRNAs in pathogenesis and early diagnosis of CE. Methods The current study evaluated the expression of four E. granulosus -derived miRNAs, including egr-miR-125,5p, egr-let-7,5p, egr-miR-2, and egr-miR-71 in fibrotic and healthy liver tissues of 31 CE patients with active and inactive hydatid cysts by qRT-PCR. Results Of the 31 patients, 48.4% had active cysts (CE1 and CE2), while the remainder had transitional (16.1%) and inactive (35.5%) CE types cysts. The qRT-PCR analysis revealed a significant increase of 11.2, 9.91, 6.2, and 13.1-fold in the fibrotic tissue group for egr-miR-125,5p, egr-let-7,5p, egr-miR-2, and egr-miR-71, respectively. Among these miRNAs, egr-miR-125-5p exhibited the highest area under the curve (AUC) value of 0.8050 for predicting liver fibrosis. Conclusions Our findings provide new data about the role of E. granulosus -derived miRNAs in pathogenesis of CE. The high AUC of egr-miR125,5p reflecting the possibility of using egr-miR125,5p as biomarker in CE diagnosis. Further studies on serum of CE patients are needed to confirm the potential role of circulating egr-miR-2a-3p and egr-miR-125-5p in the early diagnosis of CE.
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ISSN:1230-2821
1896-1851
DOI:10.1007/s11686-022-00654-x