Rhizobium terrae sp. nov., Isolated from an Oil-Contaminated Soil in China

• Published in: Current microbiology Vol. 77; no. 6; pp. 1117 - 1124
• Main Authors: Ruan, Zhe-pu, Cao, Wei-miao, Zhang, Xi, Liu, Jing-tian-yi, Zhu, Jian-chun, Hu, Bing, Jiang, Jian-dong
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.06.2020; Springer Nature B.V
• Subjects: Annotations; Base Composition; Biomedical and Life Sciences; Biotechnology; China; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - chemistry; Gene sequencing; Genes; Genes, Bacterial; Genome, Bacterial - genetics; Genomes; Hydrogen-Ion Concentration; Life Sciences; Microbiology; New species; Nucleic Acid Hybridization; Nucleotide sequence; Oil and Gas Fields - microbiology; Oil pollution; Phylogenetics; Phylogeny; Quinones; Quinones - chemistry; Rhizobium - chemistry; Rhizobium - classification; Rhizobium - cytology; Rhizobium - physiology; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sodium chloride; Sodium Chloride - metabolism; Soil contamination; Soil Microbiology; Soil pollution; Soils; Species Specificity; Temperature; China
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-020-01889-5

A Gram-stain-negative, facultative aerobic, non-spore-forming, non-motile, non-flagellated, rod-shaped bacterium, designated strain NAU-18 T was isolated from an oil-contaminated soil in China. Strain NAU-18 T could grow at 10–42 °C (optimum, 30 °C), at pH 5.0–8.0 (optimum, 7.0) and in the presence of 0–2.0% (w/v) NaCl (optimum, 0.5% NaCl in R2A). The predominant fatty acids were C 18:1 ω 7c (71.2%) and Summed feature 2 (5.1%), representing 76.3% of the total fatty acids. The major respiratory quinones were Q9 and Q10. The DNA G + C content of strain NAU-18 T was 61.4 mol% based on its draft genome sequence. Genome annotation of strain NAU-18 T predicted the presence of 6668 genes, of which 6588 are coding proteins and 80 are RNA genes. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NAU-18 T was a member of the genus Rhizobium and showed 96.93% (with 93.2% coverage) and 96.81% (with 100% coverage) identities with those of Neorhizobium alkalisoli CCBAU 01393 T and Rhizobium oryzicola ZYY136 T , respectively. In the phylogenetic analysis, strain NAU-18 T and R. oryzicola ZYY136 T are consistently placed in the same branch. Strain NAU-18 T represents a novel species within the genus Rhizobium , for which the name Rhizobium terrae sp. nov. is proposed, with the type strain NAU-18 T (=KCTC 62418 T  = CCTCC AB 2018075 T ).