Bifidobacterium lactis attenuates onset of inflammation in a murine model of colitis

AIM： To assess the anti-inflammatory effect of the probiotic Bifidobacterium lactis （B. lactis） in an adoptive transfer model of colitis. METHODS： Donor and recipient mice received either B. lactis or bacterial culture medium as control （deMan Rogosa Sharpe） in drinking water for one week prior to t...

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Published inWorld journal of gastroenterology : WJG Vol. 17; no. 4; pp. 459 - 469
Main Authors Philippe, David, Favre, Laurent, Foata, Francis, Adolfsson, Oskar, Perruisseau-Carrier, Genevieve, Vidal, Karine, Reuteler, Gloria, Dayer-Schneider, Johanna, Mueller, Christoph, Blum, Stéphanie
Format Journal Article
LanguageEnglish
Published United States Baishideng Publishing Group Co., Limited 28.01.2011
Subjects
Adoptive Transfer
Animals
Bifidobacterium - immunology
Biomarkers - metabolism
Body Weight
Colitis - immunology
Colitis - microbiology
Colitis - pathology
Disease Models, Animal
Feces - microbiology
Humans
Inflammation - drug therapy
Inflammation - immunology
Inflammation - microbiology
Mice
Mice, Inbred C57BL
Original
Probiotics - administration & dosage
Probiotics - therapeutic use
T-Lymphocytes - immunology
双歧杆菌
发病
小鼠
模型
炎症介质
球菌
结肠炎
调节性T细胞
Probiotics
Bifidobacterium
Inflammation
Mice
Colitis
Adoptive transfer model
Regulatory T cells
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Summary:AIM： To assess the anti-inflammatory effect of the probiotic Bifidobacterium lactis （B. lactis） in an adoptive transfer model of colitis. METHODS： Donor and recipient mice received either B. lactis or bacterial culture medium as control （deMan Rogosa Sharpe） in drinking water for one week prior to transfer of a mix of naive and regulatory T cells until sacrifice. RESULTS： All recipient mice developed signs of colonic inflammation, but a significant reduction of weight loss was observed in B. lactis-fed recipient mice compared to control mice. Moreover, a trend toward a diminution of mucosal thickness and attenuated epithelial damage was revealed. Colonic expression of pro-inflammatory and T cell markers was significantly reduced in B. lactis-fed recipient mice compared to controls. Concomitantly, forkhead box protein 3, a marker of regulatory T cells, was significantly up-regulated by B. lactis. CONCLUSION： Daily oral administration of B. lactis was able to reduce inflammatory and T cells mediators and to promote regulatory T cells specific markers in a mouse model of colitis.
Bibliography:Probiotics; Bifidobacterium; Colitis; Adoptive transfer model; Regulatory T cells; Inflammation; Mice
David Philippe, Laurent Favre, Francis Foata, Oskar Adolfsson, Genevieve Perruisseau-Carrier, Karine Vidal, Gloria Reuteler, Stéphanie Blum, Department of Nutrition and Health, Nestlé Research Centre, CH-1000 Lausanne 26, Switzerland Johanna Dayer-Schneider, Christoph Mueller, Department of Pathology, University of Bern, CH-3010 Bern, Switzerland
14-1219/R
AIM： To assess the anti-inflammatory effect of the probiotic Bifidobacterium lactis （B. lactis） in an adoptive transfer model of colitis. METHODS： Donor and recipient mice received either B. lactis or bacterial culture medium as control （deMan Rogosa Sharpe） in drinking water for one week prior to transfer of a mix of naive and regulatory T cells until sacrifice. RESULTS： All recipient mice developed signs of colonic inflammation, but a significant reduction of weight loss was observed in B. lactis-fed recipient mice compared to control mice. Moreover, a trend toward a diminution of mucosal thickness and attenuated epithelial damage was revealed. Colonic expression of pro-inflammatory and T cell markers was significantly reduced in B. lactis-fed recipient mice compared to controls. Concomitantly, forkhead box protein 3, a marker of regulatory T cells, was significantly up-regulated by B. lactis. CONCLUSION： Daily oral administration of B. lactis was able to reduce inflammatory and T cells mediators and to promote regulatory T cells specific markers in a mouse model of colitis.
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Author contributions: Philippe D and Favre L wrote the manuscript and contributed equally to this work; Philippe D, Favre L, Adolfsson O, Vidal K, Mueller C, Foata F and Blum S designed the study and participated in data analysis; Foata F, Perruisseau-Carrier G, Dayer-Schneider J and Reuteler G performed research; all authors approved the final manuscript.
Telephone: +41-21-7858967 Fax: +41-21-7858544
Correspondence to: David Philippe, PhD, Department of Nutrition and Health, Nestlé Research Centre, Vers-chez-les-Blanc, PO Box 44, CH-1000 Lausanne 26, Switzerland. david.philippe@rdls.nestle.com
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v17.i4.459