Chrysin based pyrimidine-piperazine hybrids: design, synthesis, in vitro antimicrobial and in silico E. coli topoisomerase II DNA gyrase efficacy

• Published in: Molecular diversity Vol. 28; no. 3; pp. 1377 - 1392
• Main Authors: Patel, Kajalben B., Rajani, Dhanji, Ahmad, Iqrar, Patel, Harun, Patel, Hitesh D., Kumari, Premlata
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.06.2024; Springer Nature B.V
• Subjects: Antimicrobial agents; Biochemistry; Biomedical and Life Sciences; E coli; Life Sciences; Organic Chemistry; Original Article; Pharmacy; Polymer Sciences; MD simulation; Docking; Antifungal; Antibacterial; Chrysin
• ISSN: 1381-1991; 1573-501X; 1573-501X
• DOI: 10.1007/s11030-023-10663-1

Ten chrysin-based pyrimidine-piperazine hybrids have been evaluated in vitro for antimicrobial activity against eleven bacterial and two fungal strains. All compounds 5a–j exhibited moderate to good inhibition, with MIC values ranging from 6.25 to 250 µg/ml. At 6.25 µg/ml and 12.5 µg/ml MIC values, respectively, compounds 5b and 5h demonstrated the most promising potency against E. coli, outperforming ampicillin, chloramphenicol, and ciprofloxacin. None of the substances had the same level of action as norfloxacin. 5a, 5d, 5g, 5h, and 5i have exhibited superior antifungal efficacy than Griseofulvin against C. albicans with 250 µg/ml MIC. All the compounds were also individually docked into the E. coli DNA gyrase ATP binding site (PDB ID: 1KZN) and CYP51 inhibitor (PDB ID: 5V5Z). The most active compound, 5h and 5g displayed a Glide docking score of − 5.97 kcal/mol and − 10.99 kcal/mol against DNA gyrase and 14α-demethylase enzyme CYP51 respectively. Potent compounds 5b, 5h, and 5g may be used to design new, innovative antimicrobial agents, according to in vitro, ADMET, and in silico biological efficacy analyses. Graphical abstract