Norovirus strains in patients with acute gastroenteritis in rural and low-income urban areas in northern Brazil

• Published in: Archives of virology Vol. 166; no. 3; pp. 905 - 913
• Main Authors: Tinker, Rory J., da Costa, Antonio Charlys, Tahmasebi, Roozbeh, Milagres, Flavio Augusto de Pádua, dos Santos Morais, Vanessa, Pandey, Ramendra Pati, José-Abrego, Alexis, Brustulin, Rafael, Rodrigues Teles, Maria da Aparecida, Cunha, Mariana Sequetin, Araújo, Emerson Luiz Lima, Gómez, Mariela Martínez, Deng, Xutao, Delwart, Eric, Sabino, Ester Cerdeira, Leal, Elcio, Luchs, Adriana
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.03.2021; Springer Nature B.V
• Subjects: Base Sequence; Biomedical and Life Sciences; Biomedicine; Brazil - epidemiology; Brief Report; Caliciviridae Infections - epidemiology; Cross-Sectional Studies; Feces - virology; Gastroenteritis; Gastroenteritis - epidemiology; Gastroenteritis - virology; Genetic diversity; Genetic Variation - genetics; Genotype; Genotypes; High-Throughput Nucleotide Sequencing; Humans; Infectious Diseases; Low income groups; Medical Microbiology; Molecular Epidemiology; Next-generation sequencing; Norovirus; Norovirus - classification; Norovirus - genetics; Norovirus - isolation & purification; Phylogeny; Poverty - statistics & numerical data; RNA, Viral - genetics; Strains (organisms); Urban areas; Virology; Brazil
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-020-04944-5

From 2010-2016, a total of 251 stool samples were screened for norovirus using next-generation sequencing (NGS) followed by phylogenetic analysis to investigate the genotypic diversity of noroviruses in rural and low-income urban areas in northern Brazil. Norovirus infection was detected in 19.9% (50/251) of the samples. Eight different genotypes were identified: GII.4_Sydney[P31] (64%, 32/50), GII.6[P7] (14%, 7/50), GII.17[P17] (6%, 3/50), GII.1[P33] (6%, 3/50), GII.3[P16] (4%, 2/50), GII.2[P16] (2%, 1/50), GII.2[P2] (2%, 1/50), and GII.4_New Orleans[P4] (2%, 1/50). Distinct GII.6[P7] variants were recognized, indicating the presence of different co-circulating strains. Elucidating norovirus genetic diversity will improve our understanding of their potential health burden, in particular for the GII.4_Sydney[P31] variant.