Effects of uridine and nucleotides on hemostasis parameters

• Published in: Journal of thrombosis and thrombolysis Vol. 55; no. 4; pp. 626 - 633
• Main Authors: Arı, Merve, Sağdilek, Engin, Kılınç, Evren, Cansev, Mehmet, Özlük, Kasım
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.05.2023; Springer Nature B.V
• Subjects: Adenosine Triphosphate - pharmacology; Blood; Blood platelets; Cardiology; Collagen; Collagen - pharmacology; Epinephrine; Hematology; Hemostasis; Humans; Medicine; Medicine & Public Health; Nucleotides; Nucleotides - pharmacology; Platelet Aggregation; Purine receptors; Uridine; Uridine - pharmacology; Uridine Diphosphate - pharmacology; Uridine monophosphate; Uridine Monophosphate - pharmacology; Uridine Triphosphate - pharmacology; UDP; UTP; Aggregometer; Platelets; Thromboelastogram
• ISSN: 1573-742X; 0929-5305; 1573-742X
• DOI: 10.1007/s11239-023-02793-y

Several purinergic receptors have been identified on platelets which are involved in hemostatic and thrombotic processes. The aim of the present study was to investigate the effects of uridine and its nucleotides on platelet aggregation and hemostasis in platelet-rich plasma (PRP) and whole blood. The effects of uridine, UMP, UDP, and UTP at different final concentrations (1 to 1000 µM) on platelet aggregation were studied using an aggregometer. In PRP samples, platelet aggregation was induced by ADP, collagen and epinephrine 3 min after addition of uridine, UMP, UDP, UTP and saline (as a control). All thromboelastogram experiments were performed at 1000 µM final concentrations of uridine and its nucleotides in whole blood. UDP and UTP were also tested in thromboelastogram with PRP. Our results showed that UDP, and especially UTP, inhibited ADP- and collagen-induced aggregation in a concentration-dependent manner. In whole blood thromboelastogram experiments, UDP stimulated clot formation while UTP suppressed clot formation. When thromboelastogram experiments were repeated with PRP, UTP’s inhibitory effect on platelets was confirmed, while UDP’s stimulated clot forming effect disappeared. Collectively, our data showed that UTP inhibited platelet aggregation in a concentration-dependent manner and suppressed clot formation. On the other hand, UDP exhibited distinct effects on whole blood or PRP in thromboelastogram. These data suggest that the difference on effects of UTP and UDP might have arisen from the different receptors that they stimulate and warrant further investigation with regard to their in vivo actions on platelet aggregation and hemostasis. Highlights UDP and UTP exhibit different effects on platelet aggregation and hemostasis. UTP inhibits platelet aggregation and hemostasis both in whole blood and PRP samples. UDP has distinct effects on hemostasis in whole blood or PRP in thromboelastogram. The findings suggest the involvement of different receptors stimulated by UDP or UTP in the observed effects.