Improving sorption performance of a molecularly imprinted monolithic column by doping mesoporous molecular sieve SBA-15

• Published in: Mikrochimica acta (1966) Vol. 189; no. 3; p. 85
• Main Authors: An, Dong-Yu, Pu, Wan-Rong, Wang, Yang, Xue-Zhang, Huang, Yan-Ping, Liu, Zhao-Sheng
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.03.2022; Springer Nature B.V
• Subjects: Adsorption; Amines; Analytical Chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Correlation coefficients; Crosslinking; Doping; Imprinted polymers; Infrared spectroscopy; Methacrylates - chemistry; Methacrylic acid; Microengineering; Molecular sieves; Molecularly Imprinted Polymers - chemistry; Nanochemistry; Nanotechnology; NMR; Nuclear magnetic resonance; Organosilicon Compounds - chemistry; Original Paper; Particle Size; Peptides; Peptides - analysis; Porosity; Recovery; Selectivity; Silicon Dioxide - chemistry; Spectrum analysis; Surface Properties; Target recognition; Solid phase extraction; Monolith column; Deep eutectic solvents; Molecularly imprinted polymer; Glutarylated peptide; Mesoporous molecular sieve
• ISSN: 0026-3672; 1436-5073; 1436-5073
• DOI: 10.1007/s00604-022-05192-x

For the first time a hybrid molecularly imprinted polymer (MIP) doped with 3-(trimethoxysilyl) propyl methacrylate (γ-MPS)-modified mesoporous molecular sieve SBA-15 for target peptide recognition has been developed. Zinc acrylate and methacrylic acid were used as binary functional monomers, and ethylene dimethacrylate was used as cross-linking agent to prepare an imprinted monolith against Val-Tyr-Ala-Leu-Lys(glutarylation) (VYALK glu ). The morphology of the polymers was characterized by scanning electron microscopy, FT-IR spectroscopy, energy dispersive spectroscopy, and 1 H NMR. The SBA-15-MPS MIP showed high recovery of 87.1% and the IF of 12.9 for the enrichment of the template peptide. When the template peptide concentration ranged from 5 to 90 μg mL −1 , the correlation coefficients ( R 2 ) for the calibration function obtained was better 0.999. The limit of detection (LOD, 0.30 μg mL −1 ) and limit of quantification (LOQ, 1.0 μg mL −1 ) were achieved for signal-to-noise ratios of 3:1 and 10:1, respectively. When other kinds of synthetic peptides were used as analogs, the selectivity of the SBA-15-MPS MIP was much better than the SBA-15-MPS NIP (without template peptides) with relative selectivity coefficients of 52.8-265. In contrast, little quinolones and biogenic amines are adsorbed with the SBA-15-MPS MIP. The SBA-15-MPS MIP could enrich VYALK glu from spiked histone digestion with the average recovery of 87.8% and the relative standard deviation (RSD) of 0.99%. As a conclusion, doping of SBA-15 is an effective approach to the improvement of performance of molecularly imprinted monolith. Graphical abstract