A Novel Flow Injection Method with Chemiluminescence Detection for the Determination of Carmoisine in Gelatin Desserts

• Published in: Journal of fluorescence Vol. 35; no. 5; pp. 3405 - 3413
• Main Authors: Abdullah, Narmin S., Hassan, Media A., Hassan, Rebwar O.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.05.2025; Springer Nature B.V
• Subjects: Analytical Chemistry; Azo Compounds - analysis; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedicine; Biophysics; Biotechnology; Chemiluminescence; Desserts; Dyes; Flow injection analysis; Flow Injection Analysis - instrumentation; Flow Injection Analysis - methods; Fluorescence; Food Coloring Agents - analysis; Gelatin; Gelatin - chemistry; Limit of Detection; Luminescence; Luminescent Measurements - instrumentation; Luminescent Measurements - methods; Naphthalenesulfonates - analysis; Reversed flow; Synthetic food; Chemiluminescence; Gelatin Desserts; Flow Injection Analysis; Carmoisine Dye
• ISSN: 1573-4994; 1053-0509; 1573-4994
• DOI: 10.1007/s10895-024-03777-8

Carmoisine dye, a red azo food colorant commonly utilized to impart a red color to synthetic food products, is the subject of this study. Here, we present a novel reversed flow injection analysis with a chemiluminescence detection (FIA-CL) method employing a newly developed homemade flow cell to determine carmoisine dye. This developed method is based on the inhibition effect of the dye on the chemiluminescence light (CL) emission generated from a luminal-hypochlorite system, whereby the reduction in CL intensity correlates directly with the concentration of carmoisine dye. Investigations into various analytical parameters were conducted to enhance method efficiency and applicability. A linear calibration graph of 4.0 to 100.0 µg mL –1 was established (R² = 0.9993), with a detection limit of LOD = 2.93 µg mL –1 . Subsequent application of the proposed method to analyze gelatine dessert samples yielded results in reasonable agreement with those obtained using the reported HPLC method, as evidenced by student t-test and F-test analyses.