An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c

An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c is described. We use a 45-min incubation in acetate buffer (pH 5.5) to eliminate labile glycated hemoglobins. In this automated system conventional modules are used but it incorporates a solvent-switching valve to...

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Published inClinical chemistry (Baltimore, Md.) Vol. 30; no. 11; pp. 1746 - 1752
Main Authors Ellis, G, Diamandis, EP, Giesbrecht, EE, Daneman, D, Allen, LC
Format Journal Article
LanguageEnglish
Published England Am Assoc Clin Chem 01.11.1984
Subjects
Adolescent
Adult
Aged
Autoanalysis
Buffers
Chromatography, High Pressure Liquid - methods
Chromatography, Ion Exchange
Female
Glycated Hemoglobin A - analysis
Hemoglobins, Abnormal - analysis
Hemolysis
Humans
Male
Middle Aged
Quality Control
Reference Values
Spectrophotometry
Temperature
Thalassemia - blood
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Summary:An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c is described. We use a 45-min incubation in acetate buffer (pH 5.5) to eliminate labile glycated hemoglobins. In this automated system conventional modules are used but it incorporates a solvent-switching valve to select either of two buffers, which differ in pH and NaCl concentration. The chromatographic column contains "polyCAT" (a weak cation-exchanger, polyaspartic acid linked to silica). Run time is 14 min per sample. The method is precise and results correlate well with those by other ion-exchange procedures.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/30.11.1746