Gold nanoclusters decorated hollow ZIF-8 encapsulating iron-catecholates as oxidase mimetics for ratiometric colorimetric detection of nitrite

• Published in: Mikrochimica acta (1966) Vol. 192; no. 1; p. 23
• Main Authors: Wu, Cunhui, Dai, Mengzhen, Qin, Xinguang, Liu, Gang, Zhang, Haizhi
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.01.2025; Springer Nature B.V
• Subjects: Analytical Chemistry; Benzidines - chemistry; Biomimetic Materials - chemistry; Catalytic activity; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Colorimetry; Colorimetry - methods; Drinking water; Encapsulation; Gold; Gold - chemistry; High resolution electron microscopy; Imidazoles - chemistry; Iron; Iron - chemistry; Limit of Detection; Metal Nanoparticles - chemistry; Microengineering; Nanochemistry; Nanoclusters; Nanotechnology; Nitrites - analysis; Oxidase; Oxidoreductases - chemistry; Photoelectrons; Sausages; Self-assembly; Transmission electron microscopy; X ray photoelectron spectroscopy; Ratiometric colorimetric detection; Photometric detection; Diazotization reaction; Nitrite; Nanozymes; Oxidase-like activity
• ISSN: 0026-3672; 1436-5073; 1436-5073
• DOI: 10.1007/s00604-024-06879-z

Gold nanoclusters decorated hollow ZIF-8 encapsulating iron-catecholates (Fe-HHTP@HZIF-8@ AuNCs) was formed through self-assembly of Fe 3+ and 2,3,6,7,10,11-hexahydroxytriphenylene (HHTP), in situ embedding of ZIF-8, and Au 3+ -Zn 2+ exchange reaction. Its morphology and structure were fully characterized by high-resolution transmission electron microscopy, X-ray diffraction, transmission electron microscopy element mapping, and X-ray photoelectron spectroscopy. Additionally, its oxidase-like activity was explored with K m of 0.21 mM and V max of 1.74 × 10 −6  M·s −1 toward 3,3′,5,5′-tetramethylbenzidine (TMB). Due to its excellent catalytic activity and nitrite mediated diazotization of oxTMB, a ratiometric colorimetric method for nitrite detection was established and validated with wide linear range (2.0–400.0 μM), low LOD (0.12 μM), high accuracy (recovery of 95.11–102.14%), and good selectivity. This method was then utilized to determine the nitrite content in sausages and tap water. This study provided a new idea for developing efficient nanozymes and offered an accurate approach for nitrite determination. Graphical Abstract