Biological activity of volatiles produced by the strains of two Pseudomonas and two Serratia species

• Published in: Folia microbiologica Vol. 68; no. 4; pp. 617 - 626
• Main Authors: Sidorova, Daria E., Khmel, Inessa A., Chernikova, Anastasya S., Chupriyanova, Tanya A., Plyuta, Vladimir A.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.08.2023; Springer Nature B.V
• Subjects: Agar; Agar - metabolism; Allelochemicals; Applied Microbiology; Bacteria; Biological activity; Biomedical and Life Sciences; Biosensors; Dextrose; E coli; Environmental Engineering/Biotechnology; Escherichia coli - metabolism; Germination; Hydrogen peroxide; Immunology; Ketones; Life Sciences; Microbiology; Microorganisms; Mixtures; Organic compounds; Original Article; Oxidative stress; Paraquat; Plant bacterial diseases; Plant growth; Pseudomonas; Pseudomonas - genetics; Pseudomonas - metabolism; Rhizosphere; Seed germination; Seeds; Serratia - genetics; Serratia - metabolism; Strain; Strains (organisms); Sulfur; Sulfur compounds; VOCs; Volatile compounds; Volatile organic compounds; Volatile Organic Compounds - pharmacology; Volatiles; Volatile organic compounds; Oxidative stress; biosensors; Volatile compounds; Seed germination; Arabidopsis thaliana; Lux-biosensors
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/s12223-023-01038-y

Volatile compounds emitted by bacteria can play a significant role in interacting with microorganisms, plants, and other organisms. In this work, we studied the effect of total gaseous mixtures of organic as well as inorganic volatile compounds (VCs) and individual pure volatile organic compounds (VOCs: ketones 2-nonanone, 2-heptanone, 2-undecanone, a sulfur-containing compound dimethyl disulfide) synthesized by the rhizosphere Pseudomonas chlororaphis 449 and Serratia plymuthica IC1270 strains, the soil-borne strain P. fluorescens B-4117, and the spoiled meat isolate S. proteamaculans 94 strain on Arabidopsis thaliana plants (on growth and germination of seeds). We demonstrated that total mixtures of volatile compounds emitted by these strains grown on Luria–Bertani agar, Tryptone Soya Agar, and Potato Dextrose Agar media inhibited the A. thaliana growth. When studied bacteria grew on Murashige and Skoog (MS) agar medium, volatile mixtures produced by bacteria could stimulate the growth of plants. Volatile compounds of bacteria slowed down the germination of plant seeds; in the presence of volatile mixtures of P. fluorescens B-4117, the seeds did not germinate. Of the individual VOCs, 2-heptanone had the most potent inhibitory effect on seed germination. We also showed that the tested VOCs did not cause oxidative stress in Escherichia coli cells using specific lux -biosensors. VOCs reduced the expression of the lux operon from the promoters of the kat G, oxy S, and sox S genes (whose products involved in the protection of cells from oxidative stress) caused by the action of hydrogen peroxide and paraquat, respectively.