Cometabolic degradation of pyrene with phenanthrene as substrate: assisted by halophilic Pseudomonas stutzeri DJP1

• Published in: Biodegradation (Dordrecht) Vol. 34; no. 6; pp. 519 - 532
• Main Authors: Jiang, Junfeng, Tian, Weijun, Lu, Zhiyang, Chu, Meile, Cao, Huimin, Zhang, Dantong
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.12.2023; Springer Nature B.V
• Subjects: Aquatic Pollution; Aromatic compounds; Aromatic hydrocarbons; Biodegradation; Biomedical and Life Sciences; Catechol; Chemical spills; Degradation; Dehydrogenases; Dioxygenase; Enzymes; Estuaries; Genes; Genomes; Geochemistry; Hydrocarbons; Life Sciences; Marine environment; Metabolites; Microbiology; Molecular weight; Oil spills; Original Paper; Phenanthrene; Phthalic acid; Pollutants; Polycyclic aromatic hydrocarbons; Pseudomonas; Pseudomonas stutzeri; Pyrene; Sediments; Soil Science & Conservation; Substrates; Terrestrial Pollution; Toxicity; Waste Management/Waste Technology; Waste Water Technology; Water Management; Water Pollution Control; Pyrene; Cometabolic degradation; Phenanthrene; Metabolic pathway; Genetic analysis
• ISSN: 0923-9820; 1572-9729
• DOI: 10.1007/s10532-023-10035-4

At present, cometabolic degradation is an extensive method for the biological removal of high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs) in the marine environment. However, due to the refractory to degradation and high toxicity, there are few studies on pyrene (PYR) cometabolic degradation with phenanthrene (PHE) as substrate. In this study, a Pseudomonas stutzeri DJP1 strain isolated from sediments was used in the cometabolic system of PHE and PYR. The biomass and the activity of key enzymes such as dehydrogenase and catechol 12 dioxygenase of strain were improved, but the enhancement of biotoxicity resulted in the inhibition of cometabolism simultaneously. Seven metabolites were identified respectively in PYR, PHE degradation cultures. It was speculated that the cometabolism of PHE and PYR had a common phthalic acid pathway, and the degradation pathway of PHE was included in the downstream pathway of PYR. The functional genes such as PhdF , NidD and CatA involved in DJP1 degradation were revealed by Genome analysis. This study provides a reference for the biodegradation of PYR and PHE in real marine environment.