Complete Genome of Bacillus velezensis CMT-6 and Comparative Genome Analysis Reveals Lipopeptide Diversity
The complete genome sequence of Bacillus velezensis type strain CMT-6 is presented for the first time. A comparative analysis between the genome sequences of CMT-6 with the genome of Bacillus amyloliquefaciens DSM7 T , B. velezensis FZB42, and Bacillus subtilis 168 revealed major differences in the...
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Published in | Biochemical genetics Vol. 58; no. 1; pp. 1 - 15 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.02.2020
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The complete genome sequence of
Bacillus velezensis
type strain CMT-6 is presented for the first time. A comparative analysis between the genome sequences of CMT-6 with the genome of
Bacillus amyloliquefaciens
DSM7
T
,
B. velezensis
FZB42, and
Bacillus subtilis
168 revealed major differences in the lipopeptide synthesis genes. Of the above, only the CMT-6 strain possessed an integrated synthetase gene for synthesizing surfactin, iturin, and fengycin. However, CMT-6 shared 14, 12, and 10 other lipopeptide-producing genes with FZB42, DSM7
T
, and 168 respectively. The largest numbers of non-synonymous mutations were detected in 205 gene sequences that produced these three lipopeptides in CMT-6 and 168. Comparing CMT-6 with DSM7
T
, 58 non-synonymous mutations were detected in gene sequences that contributed to produce lipopeptides. In addition, InDels were identified in
yczE
and
glnR
genes. CMT-6 and FZB42 had the lowest number of non-synonymous mutations with 8 lipopeptide-related gene sequences. And InDels were identified in only
yczE
. The numbers of core genes, InDels, and non-synonymous mutations in genes were the main reasons for the differences in yield and variety of lipopeptides. These results will enrich the genomic resources available for
B. velezensis
and provide fundamental information to construct strains that can produce specific lipopeptides. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-2928 1573-4927 |
DOI: | 10.1007/s10528-019-09927-z |