Immunogenicity of an inactivated novel goose parvovirus vaccine for short beak and dwarfism syndrome in Cherry Valley ducks

• Published in: Archives of virology Vol. 167; no. 3; pp. 881 - 889
• Main Authors: Zhou, Jiewen, Li, Chuanfeng, Tang, Aoxing, Li, Hang, Yu, Zhaorong, Chen, Zongyan, Guo, Xin, Liu, Guangqing
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.03.2022; Springer Nature B.V
• Subjects: Animals; Antibodies; Antibodies, Viral; Beak; Biomedical and Life Sciences; Biomedicine; Ducks; Dwarfism; Dwarfism - prevention & control; Dwarfism - veterinary; Immunization; Immunogenicity; Infectious Diseases; Medical Microbiology; Morbidity; Optical density; Original Article; Parvoviridae Infections - prevention & control; Parvoviridae Infections - veterinary; Parvovirinae; Parvoviruses; Phylogeny; Poultry Diseases - prevention & control; Vaccination; Vaccines; Vaccines, Inactivated; Viremia; Virology; Viruses; Waterfowl
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-021-05352-z

Duck short beak and dwarfism syndrome (SBDS) is a viral infectious disease caused by novel goose parvovirus (NGPV), which has been responsible for serious economic losses to the Chinese duck industry in recent years. Currently, there is no effective vaccine against this disease. In this study, we developed an inactivated virus vaccine candidate for SBDS based on NGPV strain DS15 isolated from a duck in China. Immune efficacy was evaluated in 112 ducks, which were randomly divided into vaccination, challenge-control, vaccination-challenge, and blank control groups (28 per group). Clinical characteristics, antibodies, virus excretion, viremia, and pathological changes were monitored. No morbidity or death was observed in the immunized ducks, which showed normal weight and a good mental state. High levels of serum antibodies (optical density at 450 nm of ~ 0.63) were detected in ducks immunized with the inactivated vaccine at 7 days post-vaccination (dpv), and the titer of virus-neutralizing antibodies increased from 1:2 3 to 1:2 8.5 from 7 to 42 dpv. Measurement of the viral load in anal swab, serum, and tissue samples showed that vaccination significantly inhibited the replication of NGPV in immunized ducks. Moreover, NGPV could not be isolated from the spleens of immunized or vaccinated and challenged ducks. Collectively, these results demonstrate that the newly developed inactivated NGPV vaccine, administered in an oil emulsion adjuvant, possesses good immunogenicity and represents a potentially powerful tool for SBDS prevention and control.