Harnessing chlorin e6 loaded by functionalized iron oxide nanoparticles linked with glucose for target photodynamic therapy and improving of the immunogenicity of lung cancer

• Published in: Journal of cancer research and clinical oncology Vol. 148; no. 4; pp. 867 - 879
• Main Authors: Yu, Ting-Ting, Peng, Xing-Chun, Wang, Mei-Fang, Han, Ning, Xu, Hua-Zhen, Li, Qi-Rui, Li, Liu-Gen, Xu, Xiang, Ma, Qian-Li, Liu, Bin, Wang, Jue, Zhao, Li, Chen, Xiao, Li, Tong-Fei
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.04.2022; Springer Nature B.V
• Subjects: Apoptosis; Cancer Research; Cell viability; Flow cytometry; Glucose; Hematology; HMGB1 protein; Hsp90 protein; Immunogenicity; Immunohistochemistry; Immunotherapy; Internal Medicine; Iron oxides; Light scattering; Lung cancer; Medicine; Medicine & Public Health; Microenvironments; Nanoparticles; Non-small cell lung carcinoma; Oncology; Original Article – Cancer Research; Photodynamic therapy; Reactive oxygen species; Small cell lung carcinoma; Spectrophotometry; Toxicity; Transmission electron microscopy; Tumor cells; β-Interferon; Nano-iron oxide; Photodynamic effect; Chlorin e6; Glucose; Immunogenicity; Lung cancer
• ISSN: 0171-5216; 1432-1335
• DOI: 10.1007/s00432-021-03879-x

Background Non-small-cell lung cancer (NSCLC) is the most common malignant lung tumor and is difficult to be eradicated due to its immunosuppressive microenvironment. Chlorin e6 (Ce6)-mediated photodynamic therapy (PDT) could improve immunogenicity while destroying malignant tumor cells. However, the clinic application of Ce6-mediated PDT is limited by Ce6’s poor water solubility and insufficient accumulation in lung cancer. To address this issue, Ce6 was loaded onto functionalized iron oxide nanoparticles linked with glucose to improve the distribution of Ce6 in lung cancer. Materials and results The results of transmission electron microscopy (TEM), UV–Vis spectrophotometry, dynamic light scattering and near-infrared (NIR) spectroscopy confirmed the successful preparation of the composites. Confocal and flow cytometry showed IO–PG–GLU–Ce6 significantly enhanced the uptake of Ce6 by lung cancer cells and produced more reactive oxygen species (ROS) under NIR light irradiation. In addition, the detection of cell viability, proliferation and apoptosis indicated IO–PG–GLU–Ce6 achieved stronger photo-toxicity to lung cancer cells. Moreover, IO–PG–GLU–Ce6 treatment effectively damaged the DNA of lung cancer cells and thereby activated STING, up-regulated the expression of IFN-β, HMGB1 and HSP90, indicating augmented immunogenicity of lung cancer cells. Further results of in vivo, organ imaging and tissue fluorescence sections demonstrated IO–PG–GLU–Ce6 significantly improved the distribution of Ce6 in tumor tissues of lung cancer-bearing mice as well. Finally, the findings of in vivo study and immunohistochemistry confirmed the better efficacy of IO–PG–GLU–Ce6. HE staining results of vital organs suggested that the composites were less toxic. Conclusion In conclusion, Ce6 loaded by functionalized iron oxide nanoparticles linked with glucose exhibited both target photodynamic efficacy and the ability to enhance its immunogenicity in lung cancer. This study provides a promising strategy for augment of the targeting delivery of Ce6 and its mediated photodynamic and immunotherapy.