Macrophage stimulating polysaccharide purified from peels of grape (Vitis labrusca)

• Published in: Food science and biotechnology Vol. 19; no. 2; pp. 479 - 486
• Main Authors: Kim, D.J., National Institute of Crop Science, RDA, Suwon, Republic of Korea, Lee, J.S., Chungbuk National University, Cheongju, Republic of Korea, Kim, K.J., National Institute of Crop Science, RDA, Suwon, Republic of Korea, Hong, H.C., National Institute of Crop Science, RDA, Suwon, Republic of Korea, Shin, K.S., Kyonggi University, Suwon, Republic of Korea, Yu, K.W., Chungju National University, Jeungpyeong, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society of Food Science and Technology 01.04.2010; 한국식품과학회
• Subjects: beta-glucosyl-Yariv antigen; Chemistry; Chemistry and Materials Science; Food Science; grape peel; macrophage stimulation; methylation analysis; Nutrition; pectic polysaccharide; Research Article; Vitaceae; VITIS LABRUSCA; 식품과학; grape peel; methylation analysis; β-glucosyl-Yariv antigen; pectic polysaccharide; macrophage stimulation
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-010-0067-1

To characterize the macrophage stimulating polysaccharide in grape (Vitis labrusca) peels, the active crude polysaccharide (VL-3) has been fractionated from the hot-water extract of grape peels. A macrophage stimulating polysaccharide-rich fraction (VL-3IIb-1-1) was purified from VL-3 by 3 successive column chromatographies on DEAE-Sepharose CL-6B, Sepharose CL-6B, and Sephacryl S-300. VL-3IIb-1-1 was eluted as a single peak on high performance liquid chromatography (HPLC) and its molecular weight was estimated to be 194 kDa. VL-3IIb-1-1 consisted mainly of Ara and Gal in addition to uronic acid (GalA+GlcA) (molar ratio 1.00:0.81:0.72). Methylation analysis indicated that VL-3IIb-1-1 consisted mainly of terminal Araf, 4- or 5-linked Ara, 2,4-branched Rha, 6- or 3,4- or 3,6-branched Gal, and 3,4,6-branched Glc. Single radial gel diffusion also indicated that VL-3IIb-1-1 showed an intermediate reactivity with β-glucosyl-Yariv antigen. In addition, oral administration of VL-3IIb enhanced the stimulatory responses of macrophage stimulating activity ex vivo. Therefore, VL-3IIb-1-1 purified from grape peels is suggested to be pectic polysaccharide with arabino-3,6-galactan, and it is assumed that VL-3IIb-1-1 plays an important role for expression of its activity.