CRISPR–Cas-mediated gene editing in lactic acid bacteria

• Published in: Molecular biology reports Vol. 47; no. 10; pp. 8133 - 8144
• Main Authors: Song, Xin, Zhang, Xiao-yu, Xiong, Zhi-qiang, Liu, Xin-xin, Xia, Yong-jun, Wang, Shi-jie, Ai, Lian-zhong
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.10.2020; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; CRISPR; CRISPR-Cas Systems; Gene deletion; Gene Editing; Genes, Bacterial; Genome editing; Histology; Insertion; Lactic acid; Lactic acid bacteria; Lactobacillales - genetics; Life Sciences; Morphology; Multigene Family; Point mutation; Review; CRISPR; Lactic acid bacteria; Gene editing; Gene knockout

The high efficiency, convenience and diversity of clustered regular interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems are driving a technological revolution in the gene editing of lactic acid bacteria (LAB). Cas-RNA cassettes have been adopted as tools to perform gene de...