CRISPR–Cas-mediated gene editing in lactic acid bacteria
The high efficiency, convenience and diversity of clustered regular interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems are driving a technological revolution in the gene editing of lactic acid bacteria (LAB). Cas-RNA cassettes have been adopted as tools to perform gene de...
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Published in | Molecular biology reports Vol. 47; no. 10; pp. 8133 - 8144 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.10.2020
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The high efficiency, convenience and diversity of clustered regular interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems are driving a technological revolution in the gene editing of lactic acid bacteria (LAB). Cas-RNA cassettes have been adopted as tools to perform gene deletion, insertion and point mutation in several species of LAB. In this article, we describe the basic mechanisms of the CRISPR–Cas system, and the current gene editing methods available, focusing on the CRISPR–Cas models developed for LAB. We also compare the different types of CRISPR–Cas-based genomic manipulations classified according to the different Cas proteins and the type of recombineering, and discuss the rapidly evolving landscape of CRISPR–Cas application in LAB. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 |
ISSN: | 0301-4851 1573-4978 |
DOI: | 10.1007/s11033-020-05820-w |