Lectins and ELLSA as powerful tools for glycoconjugate recognition analyses
Lectins, in combination with our established enzyme-linked lectin sorbent assay (ELLSA) and inhibition study, have been used as powerful tools in many glycoconjugate recognition studies. In this short review, we highlight the following: (i) The recognition profiles of Gal/GalNAc-specific lectins wer...
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Published in | Glycoconjugate journal Vol. 36; no. 2; pp. 175 - 183 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.04.2019
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Lectins, in combination with our established enzyme-linked lectin sorbent assay (ELLSA) and inhibition study, have been used as powerful tools in many glycoconjugate recognition studies. In this short review, we highlight the following: (i) The recognition profiles of Gal/GalNAc-specific lectins were updated and upgraded. (ii) Based on the cross-specificities of applied lectins, a new classification system was introduced. (iii) Applications of lectins for the detection and identification of
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-glycan and/or Tn glycotope in glycoconjugates were intergraded. (iv) The polyvalency of the glycotopes in glycans was found to play a critical role in glycan–lectin recognition. This is an unexplored area of glycobiology and one of the most promising directions toward the coming glycoscience transformation. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 |
ISSN: | 0282-0080 1573-4986 |
DOI: | 10.1007/s10719-019-09865-3 |