Genes for collagen types I, IV, and V are transcribed in Hela cells but a postinitiation block prevents the accumulation of type I mRNA

• Published in: Experimental cell research Vol. 192; no. 1; pp. 118 - 121
• Main Authors: Furth, John J., Wroth, Thomas H., Ackerman, Steven
• Format: Journal Article
• Language: English
• Published: Orlando, FL Elsevier Inc 1991; Elsevier
• Subjects: 550200 - Biochemistry; ADHESION; BASIC BIOLOGICAL SCIENCES; Biological and medical sciences; Cell Nucleus - metabolism; Cell-Free System; COLLAGEN; Collagen - genetics; ENZYMES; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; GENE REGULATION; GENES; HELA CELLS; Humans; MESSENGER-RNA; Molecular and cellular biology; Molecular genetics; NUCLEIC ACIDS; NUCLEOTIDYLTRANSFERASES; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; POLYMERASES; PROTEINS; RNA; RNA Polymerase II - physiology; RNA POLYMERASES; RNA Processing, Post-Transcriptional; RNA, Messenger - metabolism; SCLEROPROTEINS; TRANSCRIPTION; Transcription, Genetic - physiology; Transcription. Transcription factor. Splicing. Rna processing; TRANSFERASES; In vitro transcription; Molecular form; Messenger RNA; Transcription; Collagen; Adhesion; Hela cell line
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/0014-4827(91)90165-Q

Collagen mRNA synthesis in HeLa cells was evaluated by in vitro transcription of type I collagen DNA, nuclear run-on studies, and steady-state mRNA analysis. Type I collagen mRNA was accurately initiated by HeLa cell RNA polymerase II in nuclear extracts, and run-on analysis indicated that mRNAs for collagen types α1(I), α2(I), α1(III), α1(IV), and α2(V) were synthesized in HeLa cells. However, on assessing the steady-state levels of mRNAs of collagen types α1(I), α2(I), α1(IV), and α2(V), no type I mRNA was found in HeLa cells while types α1(IV) and α2(V) collagen mRNAs were observed. These results suggest that a postinitiation process prevents the accumulation of type I collagen mRNAs in HeLa cells. Persistence of types IV and V collagen mRNAs is consistent with the involvement of types IV and V collagen in adhesion of HeLa cells to glass or plastic.