Developmental Toxicity of Four Glycol Ethers Applied Cutaneously to Rats

• Published in: Environmental health perspectives Vol. 57; pp. 69 - 74
• Main Authors: Hardin, Bryan D., Goad, Phillip T., Burg, JeAnne R.
• Format: Journal Article
• Language: English
• Published: United States National Institute of Environmental Health Sciences. National Institutes of Health. Department of Health, Education and Welfare 01.08.1984
• Subjects: Abnormalities, Drug-Induced - etiology; Animals; Body weight; Body Weight - drug effects; Bone and Bones - abnormalities; Developmental toxicity; Embryonic and Fetal Development - drug effects; Ethers; Ethylene Glycols - toxicity; Female; Fetus; Glycols; Litter weight; Pregnancy; Rats; Symposium on Toxic Effects of Glycol Ethers, September 19-21, 1983, Cincinnati, Ohio; Teratogenicity; Uterus
• ISSN: 0091-6765; 1552-9924
• DOI: 10.1289/ehp.845769

Previous NIOSH studies demonstrated the embryo- and fetotoxicity and teratogenicity of ethylene glycol monoethyl ether (EGEE) applied to the shaved skin of pregnant rats. In the present study ethylene glycol monoethyl ether acetate (EGEEA), ethylene glycol monobutyl ether (EGBE), and diethylene glycol monoethyl ether (diEGEE) were tested in the same experimental model, using distilled water as the negative control and EGEE as a positive control. Water or undiluted glycols were applied four times daily on days 7 to 16 of gestation to the shaved interscapular skin with an automatic pipetter. Volumes of EGEE (0.25 mL), EGEEA (0.35 mL), and diEGEE (0.35 mL) were approximately equimolar (2.6 mmole per treatment). EGBE at 0.35 mL four times daily (approximately 2.7 mmole per treatment) killed 10 of 11 treated rats, and was subsequently tested at 0.12 mL (0.9 mmole) per treatment. EGEE- and EGEEA-treated rats showed a reduction in body weight relative to water controls that was associated with completely resorbed litters and significantly fewer live fetuses per litter. Fetal body weights were also significantly reduced in those groups. Visceral malformations and skeletal variations were significantly increased in EGEE and EGEEA groups over the negative control group. No embryotoxic, fetotoxic, or teratogenic effects were detected in the EGBE- or diEGEE-treated litters.