Probiotic inhibits the cytopathic effect induced by Escherichia coli O157:H7 in Vero cell line model

• Published in: Letters in applied microbiology Vol. 52; no. 5; pp. 527 - 531
• Main Authors: Tahamtan, Y, Kargar, M, Namdar, N, Rahimian, A, Hayati, M, Namavari, M.M
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.05.2011
• Subjects: Animals; bacteria; Bifidobacterium - physiology; Cell Survival - drug effects; Cercopithecus aethiops; cytopathogenicity; E. coli O157:H7; Escherichia coli; Escherichia coli Infections - microbiology; Escherichia coli O157; Microbial Interactions; milk; pathogens; probiotic; Probiotics; Shiga toxin; Shiga Toxin 1 - toxicity; Shiga Toxin 2 - toxicity; Vero cell; Vero Cells; yogurt
• ISSN: 0266-8254; 1472-765X
• DOI: 10.1111/j.1472-765X.2011.03037.x

Aims: The effectiveness of four strains of Bifidobacteria against enterohemorrhagic Escherichiacoli O157:H7 infection was studied using a Vero cell model. Methods and results: E. coli O157 was inoculated on the Vero cell line before and after treatment with probiotic. The cytopathic effect (CPE) was evaluated during 24 h of incubation. The results indicated that Shiga toxin activity was inhibited by the probiotic. To prevent a Stx2 CPE, the probiotic needs one log more than the Stx1. Conclusion: The Vero cell assay, in particular, is a good model to evaluate the effect of Bifidobacteria inhibiting bacterial attachment because of soluble substances and the competitive aspect and could be used in a variety of foods like milk and yoghurt to protect pathogen bacteria. Significance and Impact of the Study: Probiotics could control pathogenic bacteria and Vero cell introduce as a model for evaluation of probiotics against pathogen bacteria.