Frequency of specific CD8⁺ T cells for a promiscuous epitope derived from Trypanosoma cruzi KMP-11 protein in chagasic patients
The K1 peptide is a CD8⁺T cell HLA-A*0201-restricted epitope derived from the Trypanosoma cruzi KMP-11 protein. We have previously shown that this peptide induces IFN-γ secretion by CD8⁺T cells. The aim of this study was to characterize the frequency of K1-specific CD8⁺T cells in chagasic patients....
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Published in | Parasite immunology Vol. 32; no. 7; pp. 494 - 502 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Oxford, UK : Blackwell Publishing Ltd
01.07.2010
Blackwell Publishing Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | The K1 peptide is a CD8⁺T cell HLA-A*0201-restricted epitope derived from the Trypanosoma cruzi KMP-11 protein. We have previously shown that this peptide induces IFN-γ secretion by CD8⁺T cells. The aim of this study was to characterize the frequency of K1-specific CD8⁺T cells in chagasic patients. Nineteen HLA-A2⁺individuals were selected from 50 T. cruzi infected patients using flow cytometry and SSP-PCR assays. Twelve HLA-A*0201⁺noninfected donors were included as controls. Peripheral blood mononuclear cells were stained with HLA-A2-K1 tetramer, showing that 15 of 19 infected patients have K1-specific CD8⁺T cells (0·09-0·34% frequency) without differences in disease stages or severity. Of note, five of these responders were A*0205, A*0222, A*0226, A*0259 and A*0287 after molecular typing. Thus, a phenotypic and functional comparison of K1-specific CD8⁺T cells from non-HLA-A*0201 and HLA-A*0201⁺infected patients was performed. The results showed that both non-HLA-A*0201 and HLA-A*0201⁺individuals have a predominant effector memory CD8⁺T cell phenotype (CCR7-, CD62L-). Moreover, CD8⁺T cells from non-HLA-A*0201 and HLA-A*0201⁺individuals expressed IL-2, IFN-γ and perforin without any differences. These findings support that K1 peptide is a promiscuous epitope presented by HLA-A2 supertype molecules and is highly recognized by chagasic patients. |
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Bibliography: | http://dx.doi.org/10.1111/j.1365-3024.2010.01206.x Disclosures: None Financial support: Colciencias, Research Project No. 1203‐333‐18692 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0141-9838 1365-3024 |
DOI: | 10.1111/j.1365-3024.2010.01206.x |