Leaves of Raphanus sativus L. Shows Anti-Inflammatory Activity in LPS-Stimulated Macrophages via Suppression of COX-2 and iNOS Expression

• Published in: Preventive nutrition and food science Vol. 22; no. 1; pp. 50 - 55
• Main Authors: Park, Hye-Jin, Song, Minjung
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국식품영양과학회 01.03.2017; The Korean Society of Food Science and Nutrition
• Subjects: Research Note; 식품과학; anti-inflammatory; NF-κB; nitric oxide; COX-2; Raphanus sativus L
• ISSN: 2287-1098; 2287-8602
• DOI: 10.3746/pnf.2017.22.1.50

L. (RS) is a cruciferous vegetable that is widely consumed in Korea. The anticancer activity of leaves of RS (RSL) extract has been investigated; however, no studies focused on its anti-inflammatory effects. Therefore, the aim of the current study was to evaluate the anti-inflammatory effects of RSL extract. In brief, RSL powder was fractionated into -hexane, chloroform, ethyl acetate, -butanol, and water-soluble fractions. Lipopolysaccharide (LPS)-stimulated RAW264.7 cells were treated with each fraction for initial screening. It was found that the chloroform fraction significantly inhibited nitric oxide release in LPS-stimulated RAW264.7 cells with a half maximal inhibitory concentration value of 196 μg/mL. In addition, the mRNA and protein expression levels of inducible nitric oxide synthase, measured using reverse transcriptase-polymerase chain reaction and western blotting, respectively, were reduced in a concentration-dependent manner. Moreover, the inflammatory cyclooxygenase-2 enzyme expression decreased. Furthermore, the expression of nuclear factor-kappa B (NF-κB), the key regulator of the transcriptional activation of the inflammatory cytokine genes, was reduced by the RSL chloroform fraction. Therefore, the results of our study suggest that RSL exhibits anti-inflammatory effects in LPS-stimulated macrophages via NF-κB inactivation.