Distinct accessory cell requirements define two types of rat T cell hybridomas specific for unique determinants in the encephalitogenic 68- 86 region of myelin basic protein

• Published in: The Journal of immunology (1950) Vol. 144; no. 2; pp. 440 - 450
• Main Authors: Mannie, MD, Paterson, PY, Thomas, DW, Nairn, R
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 15.01.1990; American Association of Immunologists
• Subjects: 560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture; Analysis of the immune response. Humoral and cellular immunity; ANIMAL CELLS; ANIMALS; Antigen-Presenting Cells - immunology; Antigen-Presenting Cells - radiation effects; ANTIGENS; Biological and medical sciences; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL PATHWAYS; BIOLOGICAL RADIATION EFFECTS; BIOSYNTHESIS; BLOOD; BLOOD CELLS; BODY FLUIDS; CELL CULTURES; CLONE CELLS; CONNECTIVE TISSUE CELLS; DISEASES; ELECTROMAGNETIC RADIATION; ENCEPHALITIS; Encephalomyelitis, Autoimmune, Experimental - immunology; Epitopes; Fundamental and applied biological sciences. Psychology; Fundamental immunology; GAMMA RADIATION; Gamma Rays; GROWTH FACTORS; HYBRIDOMAS; Immunobiology; INFECTIOUS DISEASES; IONIZING RADIATIONS; LEUKOCYTES; Lymphocyte Activation; LYMPHOCYTES; LYMPHOKINES; MAMMALS; MATERIALS; MITOGENS; Myelin Basic Protein - immunology; NERVOUS SYSTEM DISEASES; ORGANIC COMPOUNDS; Organs and cells involved in the immune response; Peptide Fragments - immunology; PEPTIDES; PROTEINS; RADIATION EFFECTS; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT; RADIATIONS; RATS; RODENTS; SOMATIC CELLS; SPECIFICITY; Spleen - cytology; SPLEEN CELLS; SYNTHESIS; T-Lymphocytes, Helper-Inducer - immunology; VERTEBRATES; VIRAL DISEASES; Vertebrata; Mammalia; Rat; Myelin; Basic protein; Rodentia; T-Lymphocyte; Hybridoma; Encephalitogenic protein; Accessory cell; Helper cell
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.144.2.440

Six clonotypically unique T cell hybridomas from Lewis rats were used to study accessory cell activities required for class II MHC restricted T cell responses to the 68-86 encephalitogenic sequence of myelin basic protein (MBP). T cell hybrids which were cultured with GP68-86 68-86 sequence of guinea pig MBP (GPMBP) and naive splenocytes (SPL) were induced to produce IL-2 as measured by the CTLL indicator cell line. The hybrids were categorized into two subsets (designated THYB-1 and THYB-2), because two distinct subset-specific pathways of communication between accessory cells and T cells were involved in GPMBP-induced IL-2 production. These pathways were distinguished by the following six observations. First, when the duration of a pulse of SPL with GPMBP was lengthened from 1 to 4 h, these SPL lost their ability to induce IL-2 production by THYB-2 hybrids yet nevertheless retained full stimulatory activity for THYB-1 hybrids. Second, paraformaldehyde fixation of GPMBP-pulsed SPL abrogated an activity necessary for Ag-induced IL-2 production by THYB-2 hybrids. These fixed SPL were nevertheless able to stimulate THYB-1 hybrids, albeit to a lesser extent than viable unfixed SPL. Third, the addition of either cycloheximide, cytochalasin B, or 2-deoxyglucose to an Ag pulse of SPL with GPMBP dramatically inhibited the subsequent responses of THYB-2 hybrids yet had little or no effect upon the reactivity of THYB-1 hybrids. Fourth, thymocytes lacked necessary activities for GPMBP evoked IL-2 production by THYB-2 hybrids yet strongly promoted THYB-1 hybrid responses. Fifth, exposure of SPL to as little as 500 rad of gamma-irradiation markedly attenuated THYB-2 hybrid response to GPMBP but did not affect THYB-1 responses. Sixth, anti-GPMBP responses by THYB-2 hybrids were observed only in the presence of both radioresistant adherent SPL and a distinct population of radiosensitive nonadherent SPL. Conversely, THYB-1 hybrids exhibited full reactivity to GPMBP in the presence of adherent radioresistant SPL. Together, these observations reveal that two distinct accessory cell-T cell pathways mediate immune recognition of the 68-86 encephalitogenic region of MBP. Furthermore, these results indicate that subsets of Th cells can be defined by the accessory cell type-specific interactions that are necessary for Ag-mediated responses.