Micronutrient optimization results into highly improved in vitro plant regeneration in kodo (Paspalum scrobiculatum L.) and finger (Eleusine coracana (L.) Gaertn.) millets

• Published in: Plant cell, tissue and organ culture Vol. 94; no. 2; pp. 105 - 112
• Main Authors: Kothari-Chajer, Aditi, Sharma, Manju, Kachhwaha, Sumita, Kothari, S. L
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.08.2008; Springer Netherlands; Springer
• Subjects: 2,4-D; benzyladenine; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; callus; cell growth; chlorides; cobalt; copper sulfate; culture media; dose response; Eleusine coracana; Eukaryotic cell cultures; explants; Fundamental and applied biological sciences. Psychology; gibberellic acid; in vitro culture; in vitro regeneration; kinetin; Life Sciences; manganous sulfate; methodology; Methods. Procedures. Technologies; micropropagation; millets; Miscellaneous; naphthaleneacetic acid; Original Paper; Paspalum scrobiculatum; Plant cells and fungal cells; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; silver nitrate; somatic embryogenesis; zinc sulfate; Cereal tissue culture; Inorganic nutrients; Plant regeneration; Somatic embryogenesis; Monocotyledones; Tissue culture; Cereal tissue culture, Inorganic nutrients; In vitro; Optimization; Somatic embryo; Micronutrient; Regeneration; Eleusine coracana; Gramineae; Angiospermae; Nutrient; Spermatophyta; Paspalum
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-008-9392-y

Basal medium constituents and their concentration play an important role in growth and morphogenesis of plant tissues cultured in vitro. In this study effect of different inorganic nutrients (CoCl₂, MnSO₄, ZnSO₄, CuSO₄ and AgNO₃) on callus induction and plant regeneration in Paspalum scrobiculatum and Eleusine coracana was examined. A 5x and 3x increase in regeneration response at enhanced levels of CuSO₄ was noted for kodo and finger millets, respectively. Significant improvement in plant regeneration was also observed with the increase in levels of Co and Mn. Addition of AgNO₃ to the basal medium also had a stimulatory effect on callus induction and plant regeneration. Optimization of nutrient level in the basal medium has highly significant role in obtaining maximum regeneration response from explants and callus culture.