Knockdown of the AIM2 molecule attenuates ischemia-reperfusion-induced spinal neuronal pyroptosis by inhibiting AIM2 inflammasome activation and subsequent release of cleaved caspase-1 and IL-1β

• Published in: Neuropharmacology Vol. 160; p. 107661
• Main Authors: Li, Xiao-Qian, Yu, Qian, Fang, Bo, Zhang, Zai-Li, Ma, Hong
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2019
• Subjects: Absent in melanoma 2; Caspase-1; IL-1β; Inflammasome; Ischemia reperfusion injury; Pyroptosis; Inflammasome; Pyroptosis; Ischemia reperfusion injury; IL-1β; Absent in melanoma 2; Caspase-1
• ISSN: 0028-3908; 1873-7064
• DOI: 10.1016/j.neuropharm.2019.05.038

Ischemia-reperfusion (IR) injury induces activation of several inflammasomes that widely affect neuroinflammation and, subsequently, neuronal viability. The absent in melanoma 2 (AIM2) inflammasome is highly expressed in neurons after traumatic injury. This study was performed to investigate whether the AIM2 molecule acts as an initiator to trigger AIM2 inflammasome activation and regulate neuronal pyroptosis in a mouse IR model. The early motor dysfunction that occurred within the first 8 h post-IR injury was closely associated with a massive increase in dsDNA in serum and cerebrospinal fluid (CSF) at the same observed timepoints. However, the subsequent persistent dysfunction was consistent with the continuously increasing protein levels of apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC), cleaved caspase-1 and IL-1β with time. Upregulated AIM2 immunoreactivity was primarily visualized in neurons. The si-AIM2 treatment in vivo preserved part of motor function, accompanied by decreased protein levels of AIM2, ASC, cleaved caspase-1 and IL-1β. In vitro, the direct interactions between the AIM2 molecule and caspase-1 were demonstrated by immunofluorescence staining and coimmunoprecipitation. In this context, both si-AIM2 and Ac-YVAD-CMK treatments effectively maintained neuronal viability, as demonstrated by the decreased percentage of cells with pyroptosis and release of lactate dehydrogenase (LDH), accompanied by weak immunoreactivity and a decreased number of AIM2-caspase-1 positive neurons. By contrast, poly(dA-dT) treatment exacerbated neuronal pyroptosis by reversing the above-mentioned effects. However, no significant differences were observed after si-Con treatment. These results suggest AIM2 molecule played an important role in initiating AIM2 inflammasome activation through IR-induced release of ectopic dsDNA. •The absent in melanoma 2 (AIM2) inflammasome is highly expressed in spinal neurons after IR injury.•AIM2 inhibition using siRNA or Ac-YVAD prevents IR-induced neuronal damage in in vivo and in vitro IR models.•In vivo, AIM2 inhibition preserved part of motor function by decreasing AIM2 protein levels。.•In vivo, AIM2 inhibition prevented AIM2 increases and decreased caspase-1 and IL-1β cleavage.