Sensitive detection of RNA based on concatenated self-fuelled strand displacement amplification and hairpin-AgNCs

• Published in: Analytical methods Vol. 13; no. 4; pp. 447 - 452
• Main Authors: Guo, Yahui, Wang, Mofan, Shen, Fumiao, Hu, Zhigang, Ding, Hongliu, Yao, Weirong, Qian, He
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 28.01.2021
• Subjects: Amplification; DNA, Single-Stranded - genetics; Fluorescence; Metal Nanoparticles; MicroRNAs - genetics; miRNA; Nanoclusters; Signal processing; Silver; Spectrometry, Fluorescence
• ISSN: 1759-9660; 1759-9679
• DOI: 10.1039/d0ay01762k

Herein, a self-fuelled amplification strategy (SFAS) is proposed, in which two strand displacement amplification (SDA) processes were concatenated for the proliferation of ssDNA. The ssDNA then initiated a polymerase action and caused the destruction of hairpin-templated silver nanoclusters (AgNCs), resulting in decreased fluorescence for sensing miRNA-21. This SFAS-based sensor is less complicated in design and facile in operation, because of the easy concatenation of SDA and mutual enzymes used in the signal output process. The sensitivity of this SFAS-based miRNA sensor was 1.78 × 10 −11 M with a linear relationship in the range 0.02-1.0 × 10 −9 M, and the recoveries of this method ranged from 82.07% to 106.58% with an average RSD of 10.96%. Herein, a self-fuelled amplification strategy (SFAS) is proposed, in which two strand displacement amplification (SDA) processes were concatenated for the proliferation of ssDNA.