Free radical scavenging, antiproliferative activities and profiling of variations in the level of phytochemicals in different parts of broccoli (Brassica oleracea italica)

• Published in: Food chemistry Vol. 148; pp. 373 - 380
• Main Authors: Chaudhary, Ashun, Sharma, Upendra, Vig, Adarsh Pal, Singh, Bikram, Arora, Saroj
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier 01.04.2014
• Subjects: Biological and medical sciences; Brassica; Brassica - chemistry; Brassica oleracea; broccoli; cell cycle; Cell Cycle - drug effects; Cell Line; Cell Proliferation; Cell Proliferation - drug effects; chemistry; cultivars; cytotoxicity; developmental stages; drug effects; florets; Food toxicology; free radical scavengers; Free Radical Scavengers - chemistry; Free Radical Scavengers - pharmacology; free radicals; gas chromatography; Growth Inhibitors; Growth Inhibitors - chemistry; Growth Inhibitors - pharmacology; Humans; leaves; Medical sciences; pharmacology; phytochemicals; Plant Extracts; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves; Plant Leaves - chemistry; seeds; Seeds - chemistry; spectroscopy; Toxicology; Phytochemical compound; Vegetables; Glucosinolate; Variations; Glucosinolates; Cell cycle analysis; Antioxidant; Radical scavenger; Free radical; Assay; Broccoli; Brassica oleracea; Cruciferae; Dicotyledones; Angiospermae; Cell cycle; Plant origin; ROS; Spermatophyta; Antioxidant assays; Isothiocyanates; Food; PSF; palam samridhi floret; 2′,7′-dichlorofluorescin diacetate; PSL; GLs; DCFH-DA; PS3; PSS; PS5; PS7; propidium iodide; palam samridhi five days; P I; palam samridhi seeds; ITCs; palam samridhi seven days; palam samridhi leaves; palam samridhi three days
• ISSN: 0308-8146; 1873-7072; 1873-7072
• DOI: 10.1016/j.foodchem.2013.10.042

In the present study, the seeds of Broccoli (cultivar Palam samridhi) at different stages of development were being analysed for their antioxidant and antiproliferative properties. Among the antioxidant assays performed, a remarkable inhibition of superoxide radicals i.e. 94.25% observed with extracts of five days old sprout (PS5) at 2 mg/ml concentration. Although, all the extracts showed high cytotoxicity but the floret extract (PSF) found to be most effective with IC₅₀ value of 25.94 μg/ml while leaves extract (PSL) was least effective. The cell cycle analysis showed increased G₀/G₁ phase population as compare to positive control camptothecin. Profiling of various phytochemicals executed by using gas chromatography-mass spectroscopy in order to correlate the bioactivities of the extracts. A wide variation observed in the profile of GLS hydrolytic products of different extracts obtained from the seeds, sprouts (three, five and seven days), leaves and florets.